A proteomic approach provides new insights into the control of soil-borne plant pathogens by Bacillus species

Beneficial microorganisms (also known as biopesticides) are considered to be one of the most promising methods for more rational and safe crop management practices. We used Bacillus strains EU07, QST713 and FZB24, and investigated their inhibitory effect on Fusarium. Bacterial cell cultures, cell-fr...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2013-01, Vol.8 (1), p.e53182-e53182
Main Authors: Baysal, Omür, Lai, Duo, Xu, Han-Hong, Siragusa, Mirko, Calışkan, Mikail, Carimi, Francesco, da Silva, Jaime A Teixeira, Tör, Mahmut
Format: Article
Language:English
Subjects:
Agricultural production
Agriculture
Antibiotics
Bacillus
Bacillus - metabolism
Bacteria
Biodegradation
Biological Assay
Biological control
Biological Control Agents
Biology
Biopesticides
Cell walls
Control
Crop diseases
Crop management
Degradation
Food
Fusarium
Fusarium - metabolism
Fusarium oxysporum
Gene expression
Genetic Variation
Genotype
Laboratories
Lytic enzymes
Metabolism
Methods
Microorganisms
Multivariate analysis
Oil recovery
Pathogens
Peptides
Pesticides
Plant diseases
Plant Diseases - microbiology
Plant Diseases - prevention & control
Plant protection
Probiotics
Protein Folding
Protein turnover
Proteins
Proteomics - methods
Soil Microbiology
Species Specificity
Vibrio
Volatiles
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Beneficial microorganisms (also known as biopesticides) are considered to be one of the most promising methods for more rational and safe crop management practices. We used Bacillus strains EU07, QST713 and FZB24, and investigated their inhibitory effect on Fusarium. Bacterial cell cultures, cell-free supernatants and volatiles displayed varying degrees of suppressive effect. Proteomic analysis of secreted proteins from EU07 and FZB24 revealed the presence of lytic enzymes, cellulases, proteases, 1,4-β-glucanase and hydrolases, all of which contribute to degradation of the pathogen cell wall. Further proteomic investigations showed that proteins involved in metabolism, protein folding, protein degradation, translation, recognition and signal transduction cascade play an important role in the control of Fusarium oxysporum. Our findings provide new knowledge on the mechanism of action of Bacillus species and insight into biocontrol mechanisms.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0053182