Chemometric profile of root extracts of Rhodiola imbricata Edgew. with hyphenated gas chromatography mass spectrometric technique

• Published in: PloS one 2013-01, Vol.8 (1), p.e52797-e52797
• Main Authors: Tayade, Amol B, Dhar, Priyanka, Kumar, Jatinder, Sharma, Manu, Chauhan, Rajinder S, Chaurasia, Om P, Srivastava, Ravi B
• Format: Article
• Language: English
• Subjects: Acetates - chemistry; Acetic acid; Alcohols; Alkanes; Alkenes; Ammonium; Ammonium chloride; Ammonium salts; Analysis; Biological activity; Biological effects; Biology; Bis(2-ethylhexyl) phthalate; Camphor; Cell cycle; Chemistry; Chlorides; Chloroform; Chloroform - chemistry; Chromatography; Cold; Computer programs; Deserts; Design of experiments; Dichloroethane; Diketones; Dotriacontane; Drug dosages; Esters; Ethanol; Ethanol - chemistry; Ethers; Ethyl acetate; Ethylene Dichlorides - chemistry; Fatty acids; Food; Food plants; Gas chromatography; Gas Chromatography-Mass Spectrometry - methods; Halides; Heptadecane; Herbal medicine; Hexanes - chemistry; Information technology; Lauric acid; Mass spectrometry; Mass spectroscopy; Medicinal plants; Medicine; Methanol - chemistry; Methods; n-Hexane; Nervous system; Palmitic acid; Pharmaceutical Preparations - chemistry; Pharmaceutical Preparations - classification; Pharmacology; Phenols; Phytosterols; Phytotherapy; Plant Extracts - chemistry; Plant Roots - chemistry; Plants, Medicinal - chemistry; Polar environments; R&D; Research & development; Rhodiola - chemistry; Rhodiola imbricata; Saturated fatty acids; Scientific imaging; Studies; Terpenes; Tocopherol; Tocopherols; Vitamin E; Wound healing
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0052797

Rhodiola imbricata Edgew. (Rose root or Arctic root or Golden root or Shrolo), belonging to the family Crassulaceae, is an important food crop and medicinal plant in the Indian trans-Himalayan cold desert. Chemometric profile of the n-hexane, chloroform, dichloroethane, ethyl acetate, methanol, and 60% ethanol root extracts of R. imbricata were performed by hyphenated gas chromatography mass spectrometry (GC/MS) technique. GC/MS analysis was carried out using Thermo Finnigan PolarisQ Ion Trap GC/MS MS system comprising of an AS2000 liquid autosampler. Interpretation on mass spectrum of GC/MS was done using the NIST/EPA/NIH Mass Spectral Database, with NIST MS search program v.2.0g. Chemometric profile of root extracts revealed the presence of 63 phyto-chemotypes, among them, 1-pentacosanol; stigmast-5-en-3-ol, (3β,24S); 1-teracosanol; 1-henteracontanol; 17-pentatriacontene; 13-tetradecen-1-ol acetate; methyl tri-butyl ammonium chloride; bis(2-ethylhexyl) phthalate; 7,8-dimethylbenzocyclooctene; ethyl linoleate; 3-methoxy-5-methylphenol; hexadecanoic acid; camphor; 1,3-dimethoxybenzene; thujone; 1,3-benzenediol, 5-pentadecyl; benzenemethanol, 3-hydroxy, 5-methoxy; cholest-4-ene-3,6-dione; dodecanoic acid, 3-hydroxy; octadecane, 1-chloro; ethanone, 1-(4-hydroxyphenyl); α-tocopherol; ascaridole; campesterol; 1-dotriacontane; heptadecane, 9-hexyl were found to be present in major amount. Eventually, in the present study we have found phytosterols, terpenoids, fatty acids, fatty acid esters, alkyl halides, phenols, alcohols, ethers, alkanes, and alkenes as the major group of phyto-chemotypes in the different root extracts of R. imbricata. All these compounds identified by GC/MS analysis were further investigated for their biological activities and it was found that they possess a diverse range of positive pharmacological actions. In future, isolation of individual phyto-chemotypes and subjecting them to biological activity will definitely prove fruitful results in designing a novel drug.