Rapid and Targeted Introgression of Genes into Popular Wheat Cultivars Using Marker-Assisted Background Selection

A marker-assisted background selection (MABS)-based gene introgression approach in wheat (Triticum aestivum L.) was optimized, where 97% or more of a recurrent parent genome (RPG) can be recovered in just two backcross (BC) generations. A four-step MABS method was developed based on 'Plabsim�...

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Bibliographic Details
Published in:PloS one 2009-06, Vol.4 (6), p.e5752-e5752
Main Authors: Randhawa, Harpinder S, Mutti, Jasdeep S, Kidwell, Kim, Morris, Craig F, Chen, Xianming, Gill, Kulvinder S
Format: Article
Language:English
Subjects:
Agronomy
backcrossing
Biotechnology
Biotechnology/Plant Biotechnology
Carriers
Chromosome Mapping
Chromosomes
Chromosomes - ultrastructure
Computer Simulation
Crosses, Genetic
Cultivars
disease resistance
Empirical analysis
Genes
Genes, Plant
Genetic aspects
Genetic Markers
Genetic research
Genetics and Genomics
Genetics and Genomics/Bioinformatics
genome
Genome, Plant
Genomes
Genomics
Genotype
Heterozygote
introgression
marker-assisted background selection
marker-assisted selection
Markers
Mathematical models
Models, Genetic
Optimization
Oryza
Plant Biology/Agricultural Biotechnology
plant breeding
Plant Diseases - genetics
Plant pathology
Population
Probability
Progeny
Puccinia
Puccinia recondita
Recombinants
Recombination, Genetic
Rice
rust diseases
Seedlings
Simulation
simulation models
Soil sciences
Spring wheat
Stripe rust
Triticum - genetics
Triticum aestivum
Triticum dicoccum
Wheat
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Summary:A marker-assisted background selection (MABS)-based gene introgression approach in wheat (Triticum aestivum L.) was optimized, where 97% or more of a recurrent parent genome (RPG) can be recovered in just two backcross (BC) generations. A four-step MABS method was developed based on 'Plabsim' computer simulations and wheat genome structure information. During empirical optimization of this method, double recombinants around the target gene were selected in a step-wise fashion during the two BC cycles followed by selection for recurrent parent genotype on non-carrier chromosomes. The average spacing between carrier chromosome markers was
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0005752