Neuraminidase and hemagglutinin matching patterns of a highly pathogenic avian and two pandemic H1N1 influenza A viruses

• Published in: PloS one 2010-02, Vol.5 (2), p.e9167-e9167
• Main Authors: Zhang, Yonghui, Lin, Xiaojing, Wang, Guoqin, Zhou, Jianfang, Lu, Jian, Zhao, Honglan, Zhang, Fengwei, Wu, Jia, Xu, Chunqiong, Du, Ning, Li, Zi, Zhang, Ye, Wang, Xiaoyi, Bi, Shengli, Shu, Yuelong, Zhou, Hongning, Tan, Wenjie, Wu, Xiaobing, Chen, Zhihui, Wang, Yue
• Format: Article
• Language: English
• Subjects: Animals; Avian flu; Birds; Blotting, Western; Cell Line; Cell Line, Tumor; Disease Outbreaks; Epidemics; Exo-a-sialidase; Genetic engineering; Hemagglutinin Glycoproteins, Influenza Virus - genetics; Hemagglutinin Glycoproteins, Influenza Virus - metabolism; Hemagglutinins; Humans; Infectivity; Influenza; Influenza A; Influenza A Virus, H5N1 Subtype - genetics; Influenza A Virus, H5N1 Subtype - metabolism; Influenza A Virus, H5N1 Subtype - pathogenicity; Influenza in Birds - epidemiology; Influenza in Birds - virology; Influenza, Human - epidemiology; Influenza, Human - virology; Matching; Neuraminidase - genetics; Neuraminidase - metabolism; Pandemics; Reassortant Viruses - genetics; Reassortant Viruses - metabolism; Reassortant Viruses - pathogenicity; Reverse Transcriptase Polymerase Chain Reaction; RNA, Viral - genetics; RNA, Viral - metabolism; Surveying; Viral Proteins - genetics; Viral Proteins - metabolism; Virion - genetics; Virion - metabolism; Virion - pathogenicity; Virology/Emerging Viral Diseases; Virology/Host Invasion and Cell Entry; Virology/Virion Structure, Assembly, and Egress; Virulence; Viruses
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0009167

Influenza A virus displays strong reassortment characteristics, which enable it to achieve adaptation in human infection. Surveying the reassortment and virulence of novel viruses is important in the prevention and control of an influenza pandemic. Meanwhile, studying the mechanism of reassortment may accelerate the development of anti-influenza strategies. The hemagglutinin (HA) and neuraminidase (NA) matching patterns of two pandemic H1N1 viruses (the 1918 and current 2009 strains) and a highly pathogenic avian influenza A virus (H5N1) were studied using a pseudotyped particle (pp) system. Our data showed that four of the six chimeric HA/NA combinations could produce infectious pps, and that some of the chimeric pps had greater infectivity than did their ancestors, raising the possibility of reassortment among these viruses. The NA of H5N1 (A/Anhui/1/2005) could hardly reassort with the HAs of the two H1N1 viruses. Many biological characteristics of HA and NA, including infectivity, hemagglutinating ability, and NA activity, are dependent on their matching pattern. Our data suggest the existence of an interaction between HA and NA, and the HA NA matching pattern is critical for valid viral reassortment.