A safe vaccine (DV-STM-07) against Salmonella infection prevents abortion and confers protective immunity to the pregnant and new born mice

• Published in: PloS one 2010-02, Vol.5 (2), p.e9139
• Main Authors: Negi, Vidya Devi, Nagarajan, Arvindhan G, Chakravortty, Dipshikha
• Format: Article
• Language: English
• Subjects: Abortion; Abortion, Spontaneous - immunology; Abortion, Spontaneous - prevention & control; Animals; Animals, Newborn; Antibodies; Antigens; Biology; Colostrum; Congenital defects; Contamination; Cytokines; Developing countries; Enzyme-Linked Immunosorbent Assay; Female; Fetuses; Gene Expression - drug effects; Genetic disorders; Health aspects; Immune response; Immune system; Immunity; Infections; Infectious diseases; Infectious Diseases/Bacterial Infections; Infectious Diseases/Gynecologic Infections; Interferon-gamma - metabolism; Interleukin-4 - metabolism; Interleukin-6 - metabolism; Intestines - drug effects; Intestines - metabolism; Laboratories; LDCs; Lymphocytes T; Male; Maternal mortality; Medical research; Mice; Pediatrics and Child Health; Preeclampsia; Pregnancy; Pregnancy Outcome; Pregnant women; Prevention; Protein Precursors - genetics; Reverse Transcriptase Polymerase Chain Reaction; Salmonella; Salmonella enterica; Salmonella Enteritidis; Salmonella Infections, Animal - immunology; Salmonella Infections, Animal - prevention & control; Salmonella Typhimurium; Salmonella typhimurium - drug effects; Salmonella typhimurium - immunology; Salmonella Vaccines - administration & dosage; Salmonella Vaccines - immunology; Short term memory; Spleen - cytology; Spleen - metabolism; Th1 Cells - drug effects; Th1 Cells - metabolism; Th2 Cells - drug effects; Th2 Cells - metabolism; Tropical diseases; Tumor Necrosis Factor-alpha - metabolism; Typhoid; Vaccination; Vaccination - methods; Vaccines; Womens health
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0009139

Pregnancy is a transient immuno-compromised condition which has evolved to avoid the immune rejection of the fetus by the maternal immune system. The altered immune response of the pregnant female leads to increased susceptibility to invading pathogens, resulting in abortion and congenital defects of the fetus and a subnormal response to vaccination. Active vaccination during pregnancy may lead to abortion induced by heightened cell mediated immune response. In this study, we have administered the highly attenuated vaccine strain DeltapmrG-HM-D (DV-STM-07) in female mice before the onset of pregnancy and followed the immune reaction against challenge with virulent S. Typhimurium in pregnant mice. Here we demonstrate that DV-STM-07 vaccine gives protection against Salmonella in pregnant mice and also prevents Salmonella induced abortion. This protection is conferred by directing the immune response towards Th2 activation and Th1 suppression. The low Th1 response prevents abortion. The use of live attenuated vaccine just before pregnancy carries the risk of transmission to the fetus. We have shown that this vaccine is safe as the vaccine strain is quickly eliminated from the mother and is not transmitted to the fetus. This vaccine also confers immunity to the new born mice of vaccinated mothers. Since there is no evidence of the vaccine candidate reaching the new born mice, we hypothesize that it may be due to trans-colostral transfer of protective anti-Salmonella antibodies. These results suggest that our vaccine DV-STM-07 can be very useful in preventing abortion in the pregnant individuals and confer immunity to the new born. Since there are no such vaccine candidates which can be given to the new born and to the pregnant women, this vaccine holds a very bright future to combat Salmonella induced pregnancy loss.