Dissecting interferon-induced transcriptional programs in human peripheral blood cells

• Published in: PloS one 2010-03, Vol.5 (3), p.e9753
• Main Authors: Waddell, Simon J, Popper, Stephen J, Rubins, Kathleen H, Griffiths, Michael J, Brown, Patrick O, Levin, Michael, Relman, David A
• Format: Article
• Language: English
• Subjects: Acids; Biochemistry; Bioinformatics; Biological response modifiers; Blood; Blood cells; CD4 antigen; CD8 antigen; Cell activation; Cell interactions; Cluster Analysis; Cytokines; Cytokines - metabolism; DNA microarrays; DNA, Complementary - metabolism; Flow Cytometry - methods; Gene expression; Genes; Genetic aspects; Genetics and Genomics/Gene Expression; Genomes; Humans; Immune response; Immune System; Immunology; Immunology/Immune Response; Immunology/Immunity to Infections; Immunology/Innate Immunity; Immunology/Leukocyte Signaling and Gene Expression; Infections; Interferon; Interferon Type I - metabolism; Interferon-gamma - metabolism; Interferons - metabolism; Interleukin 1; Interleukin 12; Kinases; Leukemia; Leukocytes (mononuclear); Leukocytes, Mononuclear - cytology; Lymphocytes; Lymphocytes B; Lymphocytes T; Modulators; Monocytes; Monocytes - cytology; Mutation; Oligonucleotide Array Sequence Analysis; Ontology; Pathogens; Peripheral blood mononuclear cells; Populations; Proteins; Stimulation; Time Factors; Transcription; Transcription (Genetics); Transcription, Genetic; Tumor Necrosis Factor-alpha - metabolism; Tumor necrosis factor-α; Viral infections; γ-Interferon
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0009753

Interferons are key modulators of the immune system, and are central to the control of many diseases. The response of immune cells to stimuli in complex populations is the product of direct and indirect effects, and of homotypic and heterotypic cell interactions. Dissecting the global transcriptional profiles of immune cell populations may provide insights into this regulatory interplay. The host transcriptional response may also be useful in discriminating between disease states, and in understanding pathophysiology. The transcriptional programs of cell populations in health therefore provide a paradigm for deconvoluting disease-associated gene expression profiles.We used human cDNA microarrays to (1) compare the gene expression programs in human peripheral blood mononuclear cells (PBMCs) elicited by 6 major mediators of the immune response: interferons alpha, beta, omega and gamma, IL12 and TNFalpha; and (2) characterize the transcriptional responses of purified immune cell populations (CD4+ and CD8+ T cells, B cells, NK cells and monocytes) to IFNgamma stimulation. We defined a highly stereotyped response to type I interferons, while responses to IFNgamma and IL12 were largely restricted to a subset of type I interferon-inducible genes. TNFalpha stimulation resulted in a distinct pattern of gene expression. Cell type-specific transcriptional programs were identified, highlighting the pronounced response of monocytes to IFNgamma, and emergent properties associated with IFN-mediated activation of mixed cell populations. This information provides a detailed view of cellular activation by immune mediators, and contributes an interpretive framework for the definition of host immune responses in a variety of disease settings.