Host Factors Determine Anti-GM1 Response Following Oral Challenge of Chickens with Guillain-Barre Syndrome Derived Campylobacter jejuni Strain GB11

• Published in: PloS one 2010-03, Vol.5 (3), p.e9820-e9820
• Main Authors: Ang, C.W, Dijkstra, J.R, Klerk, M.A. de, Endtz, H.P, Doorn, P.A, Jacobs, B.C, Jeurissen, S.H.M, Wagenaar, J.A
• Format: Article
• Language: English
• Subjects: Adjuvants, Immunologic; Animals; Antibodies; Biosynthesis; Birds; Campylobacter; Campylobacter jejuni; Campylobacter jejuni - metabolism; Chickens; Colonization; enteritis; exchange; Experiments; G(M1) Ganglioside - metabolism; Ganglioside GM1; gangliosides; Genes; Genomes; Guillain-Barre syndrome; Guillain-Barre Syndrome - metabolism; Immunization; Immunoglobulin G; Immunoglobulin G - chemistry; Immunoglobulin M; Immunoglobulins; Immunology; Immunology/Autoimmunity; Immunology/Immunity to Infections; infection; Infections; Infectious diseases; Infectious Diseases/Bacterial Infections; Infectious Diseases/Infectious Diseases of the Nervous System; Inoculation; Kinetics; lipopolysaccharides; Lipopolysaccharides - chemistry; Medical research; Mice; Microorganisms; Microscopy; miller-fisher-syndrome; Mimicry; Molecular Mimicry; Mutagenesis; Neurology; Oligosaccharides; Pathogens; Phenols; Poultry; Proteins; Rabbits; Salmonella; Species Specificity; Studies; Veterinary medicine
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0009820

Anti-ganglioside antibodies with a pathogenic potential are present in C. jejuni-associated Guillain-Barré syndrome (GBS) patients and are probably induced by molecular mimicry. Immunization studies in rabbits and mice have demonstrated that these anti-ganglioside antibodies can be induced using purified lipo-oligosaccharides (LOS) from C. jejuni in a strong adjuvant. To investigate whether natural colonization of chickens with a ganglioside-mimicking C. jejuni strain induces an anti-ganglioside response, and to investigate the diversity in anti-ganglioside response between and within genetically different chicken lines, we orally challenged chickens with different C. jejuni strains. Oral challenge of chickens with a C. jejuni strain from a GBS patient, containing a LOS that mimics ganglioside GM1, induced specific IgM and IgG anti-LOS and anti-GM1 antibodies. Inoculation of chickens with the Penner HS:3 serostrain, without a GM1-like structure, induced anti-LOS but no anti-ganglioside antibodies. We observed different patterns of anti-LOS/ganglioside response between and within the five strains of chickens. Natural infection of chickens with C. jejuni induces anti-ganglioside antibodies. The production of antibodies is governed by both microbial and host factors.