VILIP-1 expression in vivo results in decreased mouse skin keratinocyte proliferation and tumor development

VILIP-1, a member of the neuronal Ca(2+) sensor protein family, is able to act as a tumor suppressor in carcinoma cells by inhibiting cell proliferation and migration. In order to study the role of VILIP-1 in skin carcinogenesis we generated transgenic mice overexpressing VILIP-1 in epidermis under...

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Bibliographic Details
Published in:PloS one 2010-04, Vol.5 (4), p.e10196
Main Authors: Fu, Jian, Jin, Fang, Zhang, Jirong, Fong, Kathryn, Bassi, Daniel E, Lopez De Cicco, Ricardo, Ramaraju, Divya, Braunewell, Karl-Heinz, Conti, Claudio, Benavides, Fernando, Klein-Szanto, Andres J P
Format: Article
Language:English
Subjects:
Adenosine triphosphatase
Animal models
Animals
Apoptosis
Benzo(a)pyrene
Biochemistry/Chemical Biology of the Cell
Biology
Calcium
Cancer
Carcinogenesis
Carcinogens
Carcinoma, Squamous Cell - etiology
Carcinoma, Squamous Cell - pathology
Cell Biology/Chemical Biology of the Cell
Cell Proliferation
Deoxyribonucleic acid
Disease Susceptibility
DNA
Epidermis
Gelatinase B
Genes, Tumor Suppressor
Genetic engineering
Keratin
Keratinocytes
Keratinocytes - cytology
Matrix metalloproteinase
Matrix Metalloproteinase 9
Metalloproteinase
Metastasis
Mice
Mice, Transgenic
Molecular weight
Neurocalcin
Neurocalcin - genetics
Neurocalcin - physiology
Neurons
Oncology/Skin Cancers
Pathology
Pathology/Cellular Pathology
Proteins
Pyrene
Rodents
Skin
Skin cancer
Skin Neoplasms - etiology
Skin Neoplasms - pathology
Squamous cell carcinoma
Tissue Inhibitor of Metalloproteinase-1
Transgenic animals
Transgenic mice
Tumor suppressor genes
Tumor Suppressor Proteins - genetics
Tumors
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Summary:VILIP-1, a member of the neuronal Ca(2+) sensor protein family, is able to act as a tumor suppressor in carcinoma cells by inhibiting cell proliferation and migration. In order to study the role of VILIP-1 in skin carcinogenesis we generated transgenic mice overexpressing VILIP-1 in epidermis under the control of the bovine keratin K5 promoter (K5-VILIP-1). We studied the susceptibility of FVB wild type and VILIP-1 transgenic mice to chemically mediated carcinogenesis. After 30 weeks of treatment with a two-stage carcinogenesis protocol, all animals showed numerous skin tumors. Nevertheless, K5-VILIP-1 mice showed decreased squamous cell carcinoma (SCC) multiplicity of approximately 49% (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0010196