Short-term withdrawal of mitogens prior to plating increases neuronal differentiation of human neural precursor cells

• Published in: PloS one 2009-02, Vol.4 (2), p.e4642-e4642
• Main Authors: Schwindt, Telma Tiemi, Motta, Fabiana Louise, Barnabé, Gabriela Filoso, Massant, Cristina Gonçalves, Guimarães, Alessander de Oliveira, Calcagnotto, Maria Elisa, Conceição, Fabio Silva, Pesquero, João Bosco, Rehen, Stevens, Mello, Luiz E
• Format: Article
• Language: English
• Subjects: Apoptosis; Biophysics; Cell Biology/Cell Growth and Division; Cell Biology/Neuronal and Glial Cell Biology; Cell culture; Cell Differentiation - drug effects; Cell division; Cell growth; Cell survival; Chronic illnesses; Developmental Biology/Cell Differentiation; Developmental Biology/Stem Cells; Differentiation; Epidermal growth factor; Epidermal growth factors; Fibroblast growth factor 2; Fibroblast growth factors; Gene expression; Gene Expression Profiling; Glial Fibrillary Acidic Protein - metabolism; Growth factors; Humans; Insulin-like growth factors; Intermediate Filament Proteins - metabolism; Mitogens; Mitogens - pharmacology; Nerve Tissue Proteins - metabolism; Nervous system diseases; Nestin; Neurogenesis; Neurological diseases; Neurons; Neurons - cytology; Neurons - drug effects; Neurons - metabolism; Neuroscience/Neuronal and Glial Cell Biology; Neurospheres; Physiology; Plastic properties; Plasticity; Plating; Precursors; Senescence; Stem cells; Stem Cells - cytology; Stem Cells - drug effects; Stem Cells - metabolism; Transplantation; Tubulin - metabolism
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0004642

Human neural precursor cells (hNPC) are candidates for neural transplantation in a wide range of neurological disorders. Recently, much work has been done to determine how the environment for NPC culture in vitro may alter their plasticity. Epidermal growth factor (EGF) and fibroblast growth factor-2 (FGF-2) are used to expand NPC; however, it is not clear if continuous exposure to mitogens may abrogate their subsequent differentiation. Here we evaluated if short-term removal of FGF-2 and EGF prior to plating may improve hNPC differentiation into neurons. We demonstrate that culture of neurospheres in suspension for 2 weeks without EGF-FGF-2 significantly increases neuronal differentiation and neurite extension when compared to cells cultured using standard protocols. In this condition, neurons were preferentially located in the core of the neurospheres instead of the shell. Moreover, after plating, neurons presented radial rather than randomly oriented and longer processes than controls, comprised mostly by neurons with short processes. These changes were followed by alterations in the expression of genes related to cell survival. These results show that EGF and FGF-2 removal affects NPC fate and plasticity. Taking into account that a three dimensional structure is essential for NPC differentiation, here we evaluated, for the first time, the effects of growth factors removal in whole neurospheres rather than in plated cell culture.