Several nuclear events during apoptosis depend on caspase-3 activation but do not constitute a common pathway

A number of nuclear events occur during apoptosis, including DNA laddering, nuclear lamina breakdown, phosphorylation of histones H2B and histone H2AX, and the tight binding to chromatin of HMGB1 and CAD, the nuclease responsible for DNA laddering. We have performed an epistasis analysis to investig...

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Bibliographic Details
Published in:PloS one 2009-07, Vol.4 (7), p.e6234-e6234
Main Authors: Trisciuoglio, Lisa, Bianchi, Marco Emilio
Format: Article
Language:English
Subjects:
Activation
Apoptosis
Base Sequence
Biochemistry
Breast cancer
Caspase
Caspase 3 - metabolism
Caspase 6 - metabolism
Caspase-3
Cell Biology/Cellular Death and Stress Responses
Cell Line
Cell Nucleus - enzymology
Chromatin
Chromatin - metabolism
Clusters
Cytokines
Deoxyribonucleic acid
DNA
DNA binding proteins
DNA damage
DNA fragmentation
DNA Primers
Enzyme Activation
Epistasis
Fluorescence
Genetic research
Histone H2A
Histones
Histones - metabolism
HMGB1 protein
HMGB1 Protein - metabolism
Humans
Microscopy
Molecular Biology/Chromatin Structure
Morphology
Nuclease
Nucleoproteins
Pathogenesis
Phosphorylation
Proteins
Recovery (Medical)
Rodents
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Summary:A number of nuclear events occur during apoptosis, including DNA laddering, nuclear lamina breakdown, phosphorylation of histones H2B and histone H2AX, and the tight binding to chromatin of HMGB1 and CAD, the nuclease responsible for DNA laddering. We have performed an epistasis analysis to investigate whether these events cluster together in pathways. We find that all depend directly or indirectly on caspase-3 activation. CAD activation, H2AX phosphorylation and DNA laddering cluster together into a pathway, but all other events appear to be independent of each other downstream of caspase-3, and likely evolved subject to different functional pressures.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0006234