In vivo colocalisation of oskar mRNA and trans-acting proteins revealed by quantitative imaging of the Drosophila oocyte

Efficient mRNA transport in eukaryotes requires highly orchestrated relationships between nuclear and cytoplasmic proteins. For oskar mRNA, the Drosophila posterior determinant, these spatio-temporal requirements remain opaque during its multi-step transport process. By in vivo covisualization of os...

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Bibliographic Details
Published in:PloS one 2009-07, Vol.4 (7), p.e6241-e6241
Main Authors: Mhlanga, Musa M, Bratu, Diana P, Genovesio, Auguste, Rybarska, Agata, Chenouard, Nicolas, Nehrbass, Ulf, Olivo-Marin, Jean-Christophe
Format: Article
Language:English
Subjects:
Animals
Biophysics
Cell Biology/Developmental Molecular Mechanisms
Cell division
Cellular Biology
Computational Biology/Molecular Dynamics
Cooperation
Drosophila
Drosophila melanogaster
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Eukaryotes
Gene expression
Heterogeneous-Nuclear Ribonucleoproteins - metabolism
Infections
Insects
Life Sciences
Localization
Mammals
Messenger RNA
Molecular Biology/mRNA Transport and Localization
Molecular Biology/RNA-Protein Interactions
mRNA
Mutation
Nuclei
Nuclei (cytology)
Nurses
Oligomerization
Oocytes - metabolism
Oocytes - ultrastructure
Protein transport
Proteins
Ribonucleoproteins - metabolism
RNA transport
RNA, Messenger - genetics
RNA, Messenger - metabolism
RNA-Binding Proteins - metabolism
Transport processes
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Summary:Efficient mRNA transport in eukaryotes requires highly orchestrated relationships between nuclear and cytoplasmic proteins. For oskar mRNA, the Drosophila posterior determinant, these spatio-temporal requirements remain opaque during its multi-step transport process. By in vivo covisualization of oskar mRNA with Staufen, its putative trafficking protein, we find oskar mRNA to be present in particles distinct from Staufen for part of its transport. oskar mRNA stably associated with Staufen near the posterior pole. We observe oskar mRNA to oligomerize as hundreds of copies forming large particles which are necessary for its long range transport and localization. We show the formation of these particles occurs in the nurse cell nucleus in an Hrp48-dependent manner. We present a more refined model of oskar mRNA transport in the Drosophila oocyte.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0006241