Quantitative Method to Analyze Drosophila Pupal Eye Patterning

Background: The Drosophila pupal eye has become a popular paradigm for understanding morphogenesis and tissue patterning. Correct rearrangement of cells between ommatidia is required to organize the ommatidial array across the eye field. This requires cell movement, cell death, changes to cell-cell...

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Bibliographic Details
Published in:PloS one 2009-09, Vol.4 (9), p.e7008-e7008
Main Authors: Johnson, Ruth I, Cagan, Ross L
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Body Patterning
Cell Adhesion
Cell Adhesion Molecules, Neuronal - metabolism
Cell death
cell differentiation
Cell Lineage
Cell migration
compound eyes
Cytoskeleton
Data Interpretation, Statistical
Data processing
Defects
Developmental biology
Developmental Biology/Cell Differentiation
Developmental Biology/Morphogenesis and Cell Biology
Developmental Biology/Pattern Formation
Drosophila
Drosophila - embryology
Drosophila - physiology
Drosophila melanogaster
Eye
Gene Expression Regulation, Developmental
Gene mutation
Genes
Genomes
Genotype
Genotype & phenotype
Insects
Methods
Microscopy - methods
Morphogenesis
Mutation
Ommatidia
Pattern formation
Phenotype
Photoreceptor Cells, Invertebrate - metabolism
Photoreceptors
Pupa
pupae
pupal development
Signal Transduction
Signaling
Statistical analysis
Statistical analysis of data
tissue patterning
Trends
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Summary:Background: The Drosophila pupal eye has become a popular paradigm for understanding morphogenesis and tissue patterning. Correct rearrangement of cells between ommatidia is required to organize the ommatidial array across the eye field. This requires cell movement, cell death, changes to cell-cell adhesion, signaling and fate specification. Methodology: We describe a method to quantitatively assess mis-patterning of the Drosophila pupal eye and objectively calculate a ‘mis-patterning score’ characteristic of a specific genotype. This entails step-by-step scoring of specific traits observed in pupal eyes dissected 40–42 hours after puparium formation and subsequent statistical analysis of this data. Significance: This method provides an unbiased quantitative score of mis-patterning severity that can be used to compare the impact of different genetic mutations on tissue patterning.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0007008