The DNA methylome of human peripheral blood mononuclear cells

DNA methylation plays an important role in biological processes in human health and disease. Recent technological advances allow unbiased whole-genome DNA methylation (methylome) analysis to be carried out on human cells. Using whole-genome bisulfite sequencing at 24.7-fold coverage (12.3-fold per s...

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Bibliographic Details
Published in:PLoS biology 2010-11, Vol.8 (11), p.e1000533
Main Authors: Li, Yingrui, Zhu, Jingde, Tian, Geng, Li, Ning, Li, Qibin, Ye, Mingzhi, Zheng, Hancheng, Yu, Jian, Wu, Honglong, Sun, Jihua, Zhang, Hongyu, Chen, Quan, Luo, Ruibang, Chen, Minfeng, He, Yinghua, Jin, Xin, Zhang, Qinghui, Yu, Chang, Zhou, Guangyu, Sun, Jinfeng, Huang, Yebo, Zheng, Huisong, Cao, Hongzhi, Zhou, Xiaoyu, Guo, Shicheng, Hu, Xueda, Li, Xin, Kristiansen, Karsten, Bolund, Lars, Xu, Jiujin, Wang, Wen, Yang, Huanming, Wang, Jian, Li, Ruiqiang, Beck, Stephan, Wang, Jun, Zhang, Xiuqing
Format: Article
Language:English
Subjects:
Alleles
Biotechnology
Blood
Blood cells
Classification
CpG Islands
Deoxyribonucleic acid
DNA
DNA Methylation
Gene expression
Genetic aspects
Genetics
Genetics and Genomics/Epigenetics
Genetics and Genomics/Gene Expression
Genetics and Genomics/Genomics
Genomes
Haploidy
Humans
Leukocytes, Mononuclear - metabolism
Methylation
Physiological aspects
RNA, Untranslated - genetics
Sequence Alignment
Stem cells
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Summary:DNA methylation plays an important role in biological processes in human health and disease. Recent technological advances allow unbiased whole-genome DNA methylation (methylome) analysis to be carried out on human cells. Using whole-genome bisulfite sequencing at 24.7-fold coverage (12.3-fold per strand), we report a comprehensive (92.62%) methylome and analysis of the unique sequences in human peripheral blood mononuclear cells (PBMC) from the same Asian individual whose genome was deciphered in the YH project. PBMC constitute an important source for clinical blood tests world-wide. We found that 68.4% of CpG sites and 80% displayed allele-specific expression (ASE). These data demonstrate that ASM is a recurrent phenomenon and is highly correlated with ASE in human PBMCs. Together with recently reported similar studies, our study provides a comprehensive resource for future epigenomic research and confirms new sequencing technology as a paradigm for large-scale epigenomics studies.
ISSN:1545-7885
1544-9173
1545-7885
DOI:10.1371/journal.pbio.1000533