Human erythrocytes selectively bind and enrich infectious HIV-1 virions

Although CD4(+) cells represent the major target for HIV infection in blood, claims of complement-independent binding of HIV-1 to erythrocytes and the possible role of Duffy blood group antigen, have generated controversy. To examine the question of binding to erythrocytes, HIV-1 was incubated in vi...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2009-12, Vol.4 (12), p.e8297-e8297
Main Authors: Beck, Zoltan, Brown, Bruce K, Wieczorek, Lindsay, Peachman, Kristina K, Matyas, Gary R, Polonis, Victoria R, Rao, Mangala, Alving, Carl R
Format: Article
Language:English
Subjects:
Adsorption
Analysis
Binding
Blood
Blood groups
Calcium
Cations
CD4 antigen
CD4-Positive T-Lymphocytes - metabolism
CD4-Positive T-Lymphocytes - virology
Cloning
Contamination
DC-SIGN protein
Dendritic cells
Divalent cations
Drug dosages
Erythrocyte Membrane - metabolism
Erythrocyte Membrane - virology
Erythrocytes
Erythrocytes - metabolism
Erythrocytes - virology
Ethylenediaminetetraacetic acids
Ghosts
Health aspects
Heparan sulfate
HIV
HIV infections
HIV Infections - virology
HIV-1 - isolation & purification
HIV-1 - metabolism
HIV-1 - pathogenicity
Human immunodeficiency virus
Human immunodeficiency virus 1
Humans
Immunoglobulins
Infection
Infections
Infectious diseases
Infectious Diseases/HIV Infection and AIDS
Infectivity
Leukapheresis
Leukocytes
Leukocytes - metabolism
Leukocytes - virology
Lymphocytes
Magnesium
Medical research
Medicine
Military medicine
Permissive cells
Virion - metabolism
Virions
Virology
Virology/Immunodeficiency Viruses
Virology/Persistence and Latency
Virus Attachment
Virus Internalization
Viruses
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Although CD4(+) cells represent the major target for HIV infection in blood, claims of complement-independent binding of HIV-1 to erythrocytes and the possible role of Duffy blood group antigen, have generated controversy. To examine the question of binding to erythrocytes, HIV-1 was incubated in vitro with erythrocytes from 30 healthy leukapheresis donors, and binding was determined by p24 analysis and adsorption of HIV-1 with reduction of infectivity for CD4(+) target cells. All of the cells, regardless of blood group type, bound HIV-1 p24. A typical preparation of erythrocytes bound
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0008297