Differing prevalence and diversity of bacterial species in fetal membranes from very preterm and term labor

Intrauterine infection may play a role in preterm delivery due to spontaneous preterm labor (PTL) and preterm prolonged rupture of membranes (PPROM). Because bacteria previously associated with preterm delivery are often difficult to culture, a molecular biology approach was used to identify bacteri...

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Bibliographic Details
Published in:PloS one 2009-12, Vol.4 (12), p.e8205-e8205
Main Authors: Jones, Hannah E, Harris, Kathryn A, Azizia, Malika, Bank, Lindsay, Carpenter, Bernadette, Hartley, John C, Klein, Nigel, Peebles, Donald
Format: Article
Language:English
Subjects:
Adult
Antibiotics
Bacteria
Bacteria - isolation & purification
Biodiversity
Childbirth & labor
Childrens health
Chorioamnionitis
Chorioamnionitis - pathology
Colonization
Contamination
Cytokines
Delivery (Childbirth)
Deoxyribonucleic acid
DNA
Extraembryonic Membranes - microbiology
Extraembryonic Membranes - pathology
Female
Fetal Membranes, Premature Rupture - immunology
Fetal Membranes, Premature Rupture - microbiology
Fetuses
Fibroblasts
Gynecology
Health aspects
Histocompatibility Antigens Class II - immunology
Humans
Hypertension
Immunology/Immunity to Infections
Infection
Infections
Infectious diseases
Infectious Diseases/Bacterial Infections
Inflammation - microbiology
Inflammation - pathology
Labor
Labor, Obstetric - physiology
Laboratories
Membranes
Microbiology/Medical Microbiology
Molecular biology
Monocytes
Obstetric Labor, Premature - microbiology
Obstetrics
Obstetrics/Preterm Labor
Paresis
Placenta
Placenta - microbiology
Placenta - pathology
Polymerase chain reaction
Pregnancy
Premature birth
Premature Birth - microbiology
rRNA 16S
Species diversity
Studies
Tissues
Umbilical cord
Vagina
Womens health
Young Adult
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Description
Summary:Intrauterine infection may play a role in preterm delivery due to spontaneous preterm labor (PTL) and preterm prolonged rupture of membranes (PPROM). Because bacteria previously associated with preterm delivery are often difficult to culture, a molecular biology approach was used to identify bacterial DNA in placenta and fetal membranes. We used broad-range 16S rDNA PCR and species-specific, real-time assays to amplify bacterial DNA from fetal membranes and placenta. 74 women were recruited to the following groups: PPROM
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0008205