Synthesis-dependent strand annealing in meiosis

Recent studies led to the proposal that meiotic gene conversion can result after transient engagement of the donor chromatid and subsequent DNA synthesis-dependent strand annealing (SDSA). Double Holliday junction (dHJ) intermediates were previously proposed to form both reciprocal crossover recombi...

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Bibliographic Details
Published in:PLoS biology 2007-11, Vol.5 (11), p.e299-e299
Main Authors: McMahill, Melissa S, Sham, Caroline W, Bishop, Douglas K
Format: Article
Language:English
Subjects:
Cell division
Chromosomes
Crossing Over, Genetic
Deoxyribonucleic acid
DNA
DNA Replication
DNA, Cruciform - metabolism
Genetic recombination
Genetics and Genomics
Meiosis
Models, Genetic
Recombination, Genetic
Saccharomyces cerevisiae - genetics
Yeast
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Summary:Recent studies led to the proposal that meiotic gene conversion can result after transient engagement of the donor chromatid and subsequent DNA synthesis-dependent strand annealing (SDSA). Double Holliday junction (dHJ) intermediates were previously proposed to form both reciprocal crossover recombinants (COs) and noncrossover recombinants (NCOs); however, dHJs are now thought to give rise mainly to COs, with SDSA forming most or all NCOs. To test this model in Saccharomyces cerevisiae, we constructed a random spore system in which it is possible to identify a subset of NCO recombinants that can readily be accounted for by SDSA, but not by dHJ-mediated recombination. The diagnostic class of recombinants is one in which two markers on opposite sides of a double-strand break site are converted, without conversion of an intervening heterologous insertion located on the donor chromatid. This diagnostic class represents 26% of selected NCO recombinants. Tetrad analysis using the same markers provided additional evidence that SDSA is a major pathway for NCO gene conversion in meiosis.
ISSN:1545-7885
1544-9173
1545-7885
DOI:10.1371/journal.pbio.0050299