Efferent projections of prokineticin 2 expressing neurons in the mouse suprachiasmatic nucleus

The suprachiasmatic nucleus (SCN) in the hypothalamus is the predominant circadian clock in mammals. To function as a pacemaker, the intrinsic timing signal from the SCN must be transmitted to different brain regions. Prokineticin 2 (PK2) is one of the candidate output molecules from the SCN. In thi...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2009-09, Vol.4 (9), p.e7151-e7151
Main Authors: Zhang, Chengkang, Truong, Kimberly K, Zhou, Qun-Yong
Format: Article
Language:English
Subjects:
Animals
Artificial chromosomes
Bacteria
Bacterial artificial chromosomes
Body temperature
Brain
Brain - metabolism
Chromosomes, Artificial, Bacterial
Circadian rhythm
Circadian rhythms
Cloning
Fluorescence
Gastrointestinal Hormones - biosynthesis
Gene expression
Gene Expression Regulation
Genes, Reporter
Genetic engineering
Green Fluorescent Proteins - metabolism
Hypothalamus
Hypothalamus (lateral)
Hypothalamus - metabolism
In Situ Hybridization, Fluorescence
Localization
Mice
Mice, Transgenic
Nervous system
Neurogenesis
Neurons
Neurons - metabolism
Neuropeptides - biosynthesis
Neuroscience
Neuroscience/Behavioral Neuroscience
Neuroscience/Neuronal and Glial Cell Biology
Oscillometry
Paraventricular nucleus
Pharmacology
Physiology
Polypeptides
Preoptic area
Promoter Regions, Genetic
Reporter gene
Rodents
Septum
Suprachiasmatic nucleus
Suprachiasmatic Nucleus - metabolism
Thalamus
Transgenic animals
Transgenic mice
Transplants & implants
Trends
Vasopressins - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c729t-d4a3542f4595a2f0b0665d6732894808c3a98cabf2d7124f4ab744692fae9a863
cites cdi_FETCH-LOGICAL-c729t-d4a3542f4595a2f0b0665d6732894808c3a98cabf2d7124f4ab744692fae9a863
container_end_page e7151
container_issue 9
container_start_page e7151
container_title PloS one
container_volume 4
creator Zhang, Chengkang
Truong, Kimberly K
Zhou, Qun-Yong
description The suprachiasmatic nucleus (SCN) in the hypothalamus is the predominant circadian clock in mammals. To function as a pacemaker, the intrinsic timing signal from the SCN must be transmitted to different brain regions. Prokineticin 2 (PK2) is one of the candidate output molecules from the SCN. In this study, we investigated the efferent projections of PK2-expressing neurons in the SCN through a transgenic reporter approach. Using a bacterial artificial chromosome (BAC) transgenic mouse line, in which the enhanced green fluorescence protein (EGFP) reporter gene expression was driven by the PK2 promoter, we were able to obtain an efferent projections map from the EGFP-expressing neurons in the SCN. Our data revealed that EGFP-expressing neurons in the SCN, hence representing some of the PK2-expressing neurons, projected to many known SCN target areas, including the ventral lateral septum, medial preoptic area, subparaventricular zone, paraventricular nucleus, dorsomedial hypothalamic nucleus, lateral hypothalamic area and paraventricular thalamic nucleus. The efferent projections of PK2-expressing neurons supported the role of PK2 as an output molecule of the SCN.
doi_str_mv 10.1371/journal.pone.0007151
format article
fullrecord <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1292103850</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A472859216</galeid><doaj_id>oai_doaj_org_article_7217ffb4f9d149638ae166e0695db148</doaj_id><sourcerecordid>A472859216</sourcerecordid><originalsourceid>FETCH-LOGICAL-c729t-d4a3542f4595a2f0b0665d6732894808c3a98cabf2d7124f4ab744692fae9a863</originalsourceid><addsrcrecordid>eNqNkl2L1DAYhYso7rr6D0QLguLFjPlq0twIy7LqwMKCX5eGNH0zk7FNatLK-u_N7FSdES-kF23ePuckOZyieIzRElOBX23DFL3ulkPwsEQICVzhO8UplpQsOEH07sH3SfEgpS1CFa05v1-cYClqRgU9Lb5cWgsR_FgOMWzBjC74VAa7W351HkZnnC9JCTdDhJScX5cepriD8nzcQNmHKUGZpiFqs3E69TprSj-ZDqb0sLhndZfg0fw-Kz69ufx48W5xdf12dXF-tTCCyHHRMk0rRiyrZKWJRQ3ivGq5oKSWrEa1oVrWRjeWtAITZpluBGNcEqtB6prTs-Lp3nfoQlJzNElhIglGtK5QJlZ7og16q4boeh1_qKCduh2EuFY65pN3oATBwtqGWdliJjmtNWDOAXFZtQ1mdfZ6Pe82NT20JscXdXdkevzHu41ah--KCCYQYtngxWwQw7cJ0qh6lwx0nfaQ41SCMsQpFiKTz_4i_3255Z5a63x-523I25r8tNA7kwtiXZ6fM0HqKot2eb08EmRmhJtxraeU1OrD-_9nrz8fs88P2A3obtyk0E23tToG2R40MaQUwf5ODyO16_eve6pdv9Xc7yx7cpj8H9FcaPoTsvX2rA</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1292103850</pqid></control><display><type>article</type><title>Efferent projections of prokineticin 2 expressing neurons in the mouse suprachiasmatic nucleus</title><source>Publicly Available Content Database (Proquest) (PQ_SDU_P3)</source><source>PubMed Central</source><creator>Zhang, Chengkang ; Truong, Kimberly K ; Zhou, Qun-Yong</creator><contributor>Nitabach, Michael N.</contributor><creatorcontrib>Zhang, Chengkang ; Truong, Kimberly K ; Zhou, Qun-Yong ; Nitabach, Michael N.</creatorcontrib><description>The suprachiasmatic nucleus (SCN) in the hypothalamus is the predominant circadian clock in mammals. To function as a pacemaker, the intrinsic timing signal from the SCN must be transmitted to different brain regions. Prokineticin 2 (PK2) is one of the candidate output molecules from the SCN. In this study, we investigated the efferent projections of PK2-expressing neurons in the SCN through a transgenic reporter approach. Using a bacterial artificial chromosome (BAC) transgenic mouse line, in which the enhanced green fluorescence protein (EGFP) reporter gene expression was driven by the PK2 promoter, we were able to obtain an efferent projections map from the EGFP-expressing neurons in the SCN. Our data revealed that EGFP-expressing neurons in the SCN, hence representing some of the PK2-expressing neurons, projected to many known SCN target areas, including the ventral lateral septum, medial preoptic area, subparaventricular zone, paraventricular nucleus, dorsomedial hypothalamic nucleus, lateral hypothalamic area and paraventricular thalamic nucleus. The efferent projections of PK2-expressing neurons supported the role of PK2 as an output molecule of the SCN.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0007151</identifier><identifier>PMID: 19784373</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Artificial chromosomes ; Bacteria ; Bacterial artificial chromosomes ; Body temperature ; Brain ; Brain - metabolism ; Chromosomes, Artificial, Bacterial ; Circadian rhythm ; Circadian rhythms ; Cloning ; Fluorescence ; Gastrointestinal Hormones - biosynthesis ; Gene expression ; Gene Expression Regulation ; Genes, Reporter ; Genetic engineering ; Green Fluorescent Proteins - metabolism ; Hypothalamus ; Hypothalamus (lateral) ; Hypothalamus - metabolism ; In Situ Hybridization, Fluorescence ; Localization ; Mice ; Mice, Transgenic ; Nervous system ; Neurogenesis ; Neurons ; Neurons - metabolism ; Neuropeptides - biosynthesis ; Neuroscience ; Neuroscience/Behavioral Neuroscience ; Neuroscience/Neuronal and Glial Cell Biology ; Oscillometry ; Paraventricular nucleus ; Pharmacology ; Physiology ; Polypeptides ; Preoptic area ; Promoter Regions, Genetic ; Reporter gene ; Rodents ; Septum ; Suprachiasmatic nucleus ; Suprachiasmatic Nucleus - metabolism ; Thalamus ; Transgenic animals ; Transgenic mice ; Transplants &amp; implants ; Trends ; Vasopressins - metabolism</subject><ispartof>PloS one, 2009-09, Vol.4 (9), p.e7151-e7151</ispartof><rights>COPYRIGHT 2009 Public Library of Science</rights><rights>2009 Zhang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Zhang et al. 2009</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c729t-d4a3542f4595a2f0b0665d6732894808c3a98cabf2d7124f4ab744692fae9a863</citedby><cites>FETCH-LOGICAL-c729t-d4a3542f4595a2f0b0665d6732894808c3a98cabf2d7124f4ab744692fae9a863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1292103850/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1292103850?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25751,27922,27923,37010,37011,44588,53789,53791,74896</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19784373$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Nitabach, Michael N.</contributor><creatorcontrib>Zhang, Chengkang</creatorcontrib><creatorcontrib>Truong, Kimberly K</creatorcontrib><creatorcontrib>Zhou, Qun-Yong</creatorcontrib><title>Efferent projections of prokineticin 2 expressing neurons in the mouse suprachiasmatic nucleus</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The suprachiasmatic nucleus (SCN) in the hypothalamus is the predominant circadian clock in mammals. To function as a pacemaker, the intrinsic timing signal from the SCN must be transmitted to different brain regions. Prokineticin 2 (PK2) is one of the candidate output molecules from the SCN. In this study, we investigated the efferent projections of PK2-expressing neurons in the SCN through a transgenic reporter approach. Using a bacterial artificial chromosome (BAC) transgenic mouse line, in which the enhanced green fluorescence protein (EGFP) reporter gene expression was driven by the PK2 promoter, we were able to obtain an efferent projections map from the EGFP-expressing neurons in the SCN. Our data revealed that EGFP-expressing neurons in the SCN, hence representing some of the PK2-expressing neurons, projected to many known SCN target areas, including the ventral lateral septum, medial preoptic area, subparaventricular zone, paraventricular nucleus, dorsomedial hypothalamic nucleus, lateral hypothalamic area and paraventricular thalamic nucleus. The efferent projections of PK2-expressing neurons supported the role of PK2 as an output molecule of the SCN.</description><subject>Animals</subject><subject>Artificial chromosomes</subject><subject>Bacteria</subject><subject>Bacterial artificial chromosomes</subject><subject>Body temperature</subject><subject>Brain</subject><subject>Brain - metabolism</subject><subject>Chromosomes, Artificial, Bacterial</subject><subject>Circadian rhythm</subject><subject>Circadian rhythms</subject><subject>Cloning</subject><subject>Fluorescence</subject><subject>Gastrointestinal Hormones - biosynthesis</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Genes, Reporter</subject><subject>Genetic engineering</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>Hypothalamus</subject><subject>Hypothalamus (lateral)</subject><subject>Hypothalamus - metabolism</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Localization</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>Nervous system</subject><subject>Neurogenesis</subject><subject>Neurons</subject><subject>Neurons - metabolism</subject><subject>Neuropeptides - biosynthesis</subject><subject>Neuroscience</subject><subject>Neuroscience/Behavioral Neuroscience</subject><subject>Neuroscience/Neuronal and Glial Cell Biology</subject><subject>Oscillometry</subject><subject>Paraventricular nucleus</subject><subject>Pharmacology</subject><subject>Physiology</subject><subject>Polypeptides</subject><subject>Preoptic area</subject><subject>Promoter Regions, Genetic</subject><subject>Reporter gene</subject><subject>Rodents</subject><subject>Septum</subject><subject>Suprachiasmatic nucleus</subject><subject>Suprachiasmatic Nucleus - metabolism</subject><subject>Thalamus</subject><subject>Transgenic animals</subject><subject>Transgenic mice</subject><subject>Transplants &amp; implants</subject><subject>Trends</subject><subject>Vasopressins - metabolism</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl2L1DAYhYso7rr6D0QLguLFjPlq0twIy7LqwMKCX5eGNH0zk7FNatLK-u_N7FSdES-kF23ePuckOZyieIzRElOBX23DFL3ulkPwsEQICVzhO8UplpQsOEH07sH3SfEgpS1CFa05v1-cYClqRgU9Lb5cWgsR_FgOMWzBjC74VAa7W351HkZnnC9JCTdDhJScX5cepriD8nzcQNmHKUGZpiFqs3E69TprSj-ZDqb0sLhndZfg0fw-Kz69ufx48W5xdf12dXF-tTCCyHHRMk0rRiyrZKWJRQ3ivGq5oKSWrEa1oVrWRjeWtAITZpluBGNcEqtB6prTs-Lp3nfoQlJzNElhIglGtK5QJlZ7og16q4boeh1_qKCduh2EuFY65pN3oATBwtqGWdliJjmtNWDOAXFZtQ1mdfZ6Pe82NT20JscXdXdkevzHu41ah--KCCYQYtngxWwQw7cJ0qh6lwx0nfaQ41SCMsQpFiKTz_4i_3255Z5a63x-523I25r8tNA7kwtiXZ6fM0HqKot2eb08EmRmhJtxraeU1OrD-_9nrz8fs88P2A3obtyk0E23tToG2R40MaQUwf5ODyO16_eve6pdv9Xc7yx7cpj8H9FcaPoTsvX2rA</recordid><startdate>20090928</startdate><enddate>20090928</enddate><creator>Zhang, Chengkang</creator><creator>Truong, Kimberly K</creator><creator>Zhou, Qun-Yong</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20090928</creationdate><title>Efferent projections of prokineticin 2 expressing neurons in the mouse suprachiasmatic nucleus</title><author>Zhang, Chengkang ; Truong, Kimberly K ; Zhou, Qun-Yong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c729t-d4a3542f4595a2f0b0665d6732894808c3a98cabf2d7124f4ab744692fae9a863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Artificial chromosomes</topic><topic>Bacteria</topic><topic>Bacterial artificial chromosomes</topic><topic>Body temperature</topic><topic>Brain</topic><topic>Brain - metabolism</topic><topic>Chromosomes, Artificial, Bacterial</topic><topic>Circadian rhythm</topic><topic>Circadian rhythms</topic><topic>Cloning</topic><topic>Fluorescence</topic><topic>Gastrointestinal Hormones - biosynthesis</topic><topic>Gene expression</topic><topic>Gene Expression Regulation</topic><topic>Genes, Reporter</topic><topic>Genetic engineering</topic><topic>Green Fluorescent Proteins - metabolism</topic><topic>Hypothalamus</topic><topic>Hypothalamus (lateral)</topic><topic>Hypothalamus - metabolism</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Localization</topic><topic>Mice</topic><topic>Mice, Transgenic</topic><topic>Nervous system</topic><topic>Neurogenesis</topic><topic>Neurons</topic><topic>Neurons - metabolism</topic><topic>Neuropeptides - biosynthesis</topic><topic>Neuroscience</topic><topic>Neuroscience/Behavioral Neuroscience</topic><topic>Neuroscience/Neuronal and Glial Cell Biology</topic><topic>Oscillometry</topic><topic>Paraventricular nucleus</topic><topic>Pharmacology</topic><topic>Physiology</topic><topic>Polypeptides</topic><topic>Preoptic area</topic><topic>Promoter Regions, Genetic</topic><topic>Reporter gene</topic><topic>Rodents</topic><topic>Septum</topic><topic>Suprachiasmatic nucleus</topic><topic>Suprachiasmatic Nucleus - metabolism</topic><topic>Thalamus</topic><topic>Transgenic animals</topic><topic>Transgenic mice</topic><topic>Transplants &amp; implants</topic><topic>Trends</topic><topic>Vasopressins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Chengkang</creatorcontrib><creatorcontrib>Truong, Kimberly K</creatorcontrib><creatorcontrib>Zhou, Qun-Yong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale in Context : Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Proquest Nursing &amp; Allied Health Source</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological &amp; Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health Medical collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>Advanced Technologies &amp; Aerospace Database‎ (1962 - current)</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Meteorological &amp; Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>Biological Sciences</collection><collection>Agriculture Science Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>ProQuest advanced technologies &amp; aerospace journals</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Chengkang</au><au>Truong, Kimberly K</au><au>Zhou, Qun-Yong</au><au>Nitabach, Michael N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Efferent projections of prokineticin 2 expressing neurons in the mouse suprachiasmatic nucleus</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2009-09-28</date><risdate>2009</risdate><volume>4</volume><issue>9</issue><spage>e7151</spage><epage>e7151</epage><pages>e7151-e7151</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The suprachiasmatic nucleus (SCN) in the hypothalamus is the predominant circadian clock in mammals. To function as a pacemaker, the intrinsic timing signal from the SCN must be transmitted to different brain regions. Prokineticin 2 (PK2) is one of the candidate output molecules from the SCN. In this study, we investigated the efferent projections of PK2-expressing neurons in the SCN through a transgenic reporter approach. Using a bacterial artificial chromosome (BAC) transgenic mouse line, in which the enhanced green fluorescence protein (EGFP) reporter gene expression was driven by the PK2 promoter, we were able to obtain an efferent projections map from the EGFP-expressing neurons in the SCN. Our data revealed that EGFP-expressing neurons in the SCN, hence representing some of the PK2-expressing neurons, projected to many known SCN target areas, including the ventral lateral septum, medial preoptic area, subparaventricular zone, paraventricular nucleus, dorsomedial hypothalamic nucleus, lateral hypothalamic area and paraventricular thalamic nucleus. The efferent projections of PK2-expressing neurons supported the role of PK2 as an output molecule of the SCN.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>19784373</pmid><doi>10.1371/journal.pone.0007151</doi><tpages>e7151</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1932-6203
ispartof PloS one, 2009-09, Vol.4 (9), p.e7151-e7151
issn 1932-6203
1932-6203
language eng
recordid cdi_plos_journals_1292103850
source Publicly Available Content Database (Proquest) (PQ_SDU_P3); PubMed Central
subjects Animals
Artificial chromosomes
Bacteria
Bacterial artificial chromosomes
Body temperature
Brain
Brain - metabolism
Chromosomes, Artificial, Bacterial
Circadian rhythm
Circadian rhythms
Cloning
Fluorescence
Gastrointestinal Hormones - biosynthesis
Gene expression
Gene Expression Regulation
Genes, Reporter
Genetic engineering
Green Fluorescent Proteins - metabolism
Hypothalamus
Hypothalamus (lateral)
Hypothalamus - metabolism
In Situ Hybridization, Fluorescence
Localization
Mice
Mice, Transgenic
Nervous system
Neurogenesis
Neurons
Neurons - metabolism
Neuropeptides - biosynthesis
Neuroscience
Neuroscience/Behavioral Neuroscience
Neuroscience/Neuronal and Glial Cell Biology
Oscillometry
Paraventricular nucleus
Pharmacology
Physiology
Polypeptides
Preoptic area
Promoter Regions, Genetic
Reporter gene
Rodents
Septum
Suprachiasmatic nucleus
Suprachiasmatic Nucleus - metabolism
Thalamus
Transgenic animals
Transgenic mice
Transplants & implants
Trends
Vasopressins - metabolism
title Efferent projections of prokineticin 2 expressing neurons in the mouse suprachiasmatic nucleus
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-13T17%3A12%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Efferent%20projections%20of%20prokineticin%202%20expressing%20neurons%20in%20the%20mouse%20suprachiasmatic%20nucleus&rft.jtitle=PloS%20one&rft.au=Zhang,%20Chengkang&rft.date=2009-09-28&rft.volume=4&rft.issue=9&rft.spage=e7151&rft.epage=e7151&rft.pages=e7151-e7151&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0007151&rft_dat=%3Cgale_plos_%3EA472859216%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c729t-d4a3542f4595a2f0b0665d6732894808c3a98cabf2d7124f4ab744692fae9a863%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1292103850&rft_id=info:pmid/19784373&rft_galeid=A472859216&rfr_iscdi=true