Cdk5 is involved in BDNF-stimulated dendritic growth in hippocampal neurons

Neurotrophins are key regulators of neuronal survival and differentiation during development. Activation of their cognate receptors, Trk receptors, a family of receptor tyrosine kinases (RTKs), is pivotal for mediating the downstream functions of neurotrophins. Recent studies reveal that cyclin-depe...

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Bibliographic Details
Published in:PLoS biology 2007-04, Vol.5 (4), p.e63-e63
Main Authors: Cheung, Zelda H, Chin, Wing Hong, Chen, Yu, Ng, Yu Pong, Ip, Nancy Y
Format: Article
Language:English
Subjects:
Animals
Arthropods
Base Sequence
Brain-derived neurotrophic factor
Brain-Derived Neurotrophic Factor - physiology
Cell Biology
Cercopithecus aethiops
COS Cells
Cyclic adenylic acid
Cyclin-Dependent Kinase 5 - metabolism
Cyclin-Dependent Kinase 5 - physiology
Cyclin-dependent kinases
Dendrites
Dendritic cells
Hippocampus - cytology
Mammals
Mus (Mouse)
Neurons
Neurons - cytology
Neuroscience
Phosphorylation
Protein kinases
Proteins
Receptor, trkB - chemistry
Receptor, trkB - metabolism
RNA Interference
Rodents
Substrate Specificity
Vertebrates
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Summary:Neurotrophins are key regulators of neuronal survival and differentiation during development. Activation of their cognate receptors, Trk receptors, a family of receptor tyrosine kinases (RTKs), is pivotal for mediating the downstream functions of neurotrophins. Recent studies reveal that cyclin-dependent kinase 5 (Cdk5), a serine/threonine kinase, may modulate RTK signaling through phosphorylation of the receptor. Given the abundant expression of both Cdk5 and Trk receptors in the nervous system, and their mutual involvement in the regulation of neuronal architecture and synaptic functions, it is of interest to investigate if Cdk5 may also modulate Trk signaling. In the current study, we report the identification of TrkB as a Cdk5 substrate. Cdk5 phosphorylates TrkB at Ser478 at the intracellular juxtamembrane region of TrkB. Interestingly, attenuation of Cdk5 activity or overexpression of a TrkB mutant lacking the Cdk5 phosphorylation site essentially abolishes brain-derived neurotrophic factor (BDNF)-triggered dendritic growth in primary hippocampal neurons. In addition, we found that Cdk5 is involved in BDNF-induced activation of Rho GTPase Cdc42, which is essential for BDNF-triggered dendritic growth. Our observations therefore reveal an unanticipated role of Cdk5 in TrkB-mediated regulation of dendritic growth through modulation of BDNF-induced Cdc42 activation.
ISSN:1545-7885
1544-9173
1545-7885
DOI:10.1371/journal.pbio.0050063