A shortcut to immortality: rapid reprogramming with tissue cells

Infecting human skin cells (or mouse embryonic fibroblasts, MEFs, in the 2006 study) with retroviral vectors carrying four reprogramming genes--Oct3/4, Sox2, Klf4, and c-Myc--generated induced pluripotent stem (iPS) cells that appear indistinguishable from ES cells in terms of morphology, cell surfa...

Full description

Saved in:
Bibliographic Details
Published in:PLoS biology 2008-10, Vol.6 (10), p.e275-e275
Main Author: Gross, Liza
Format: Article
Language:English
Subjects:
Cell Biology
Developmental Biology
Embryos
Epigenetics
Stem cells
Studies
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Infecting human skin cells (or mouse embryonic fibroblasts, MEFs, in the 2006 study) with retroviral vectors carrying four reprogramming genes--Oct3/4, Sox2, Klf4, and c-Myc--generated induced pluripotent stem (iPS) cells that appear indistinguishable from ES cells in terms of morphology, cell surface markers, proliferative capacity, gene expression, and methylation state. Because this technique requires fewer viral integrations, it may reduce the risk of cancer-promoting transgene integrations, a concern for using human iPS cells in regenerative medicine.
ISSN:1545-7885
1544-9173
1545-7885
DOI:10.1371/journal.pbio.0060275