PER-TIM interactions with the photoreceptor cryptochrome mediate circadian temperature responses in Drosophila

Drosophila cryptochrome (CRY) is a key circadian photoreceptor that interacts with the period and timeless proteins (PER and TIM) in a light-dependent manner. We show here that a heat pulse also mediates this interaction, and heat-induced phase shifts are severely reduced in the cryptochrome loss-of...

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Bibliographic Details
Published in:PLoS biology 2007-06, Vol.5 (6), p.e146-e146
Main Authors: Kaushik, Rachna, Nawathean, Pipat, Busza, Ania, Murad, Alejandro, Emery, Patrick, Rosbash, Michael
Format: Article
Language:English
Subjects:
Animals
Behavior
Behavior, Animal
Biological Clocks - genetics
Biological Clocks - physiology
Biological research
Biology, Experimental
Circadian rhythm
Circadian Rhythm - genetics
Circadian Rhythm - physiology
Circadian rhythms
Cryptochromes
DNA repair
Drosophila
Drosophila - genetics
Drosophila - metabolism
Drosophila Proteins - metabolism
Experiments
Flavoproteins - genetics
Flavoproteins - metabolism
Gene expression
Heat
Hot Temperature
Influence
Insects
Kinases
Light
Models, Biological
Mutation
Neuroscience
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Period Circadian Proteins
Photoreceptors
Physiological aspects
Proteins
Temperature
Time Factors
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Summary:Drosophila cryptochrome (CRY) is a key circadian photoreceptor that interacts with the period and timeless proteins (PER and TIM) in a light-dependent manner. We show here that a heat pulse also mediates this interaction, and heat-induced phase shifts are severely reduced in the cryptochrome loss-of-function mutant cry(b). The period mutant per(L) manifests a comparable CRY dependence and dramatically enhanced temperature sensitivity of biochemical interactions and behavioral phase shifting. Remarkably, CRY is also critical for most of the abnormal temperature compensation of per(L) flies, because a per(L); cry(b) strain manifests nearly normal temperature compensation. Finally, light and temperature act together to affect rhythms in wild-type flies. The results indicate a role for CRY in circadian temperature as well as light regulation and suggest that these two features of the external 24-h cycle normally act together to dictate circadian phase.
ISSN:1545-7885
1544-9173
1545-7885
DOI:10.1371/journal.pbio.0050146