Histone H1 subtypes differentially modulate chromatin condensation without preventing ATP-dependent remodeling by SWI/SNF or NURF

• Published in: PloS one 2009-10, Vol.4 (10), p.e0007243-e0007243
• Main Authors: Clausell, Jaime, Happel, Nicole, Hale, Tracy K, Doenecke, Detlef, Beato, Miguel
• Format: Article
• Language: English
• Subjects: Adenosine Triphosphate - chemistry; Animals; Atomic force microscopy; ATP; Biochemistry; Biochemistry/Macromolecular Assemblies and Machines; Biophysics/Structural Genomics; Chimeras; Chromatin; Chromatin - chemistry; Chromatin Assembly and Disassembly; Chromatin remodeling; Chromosomal Proteins, Non-Histone - chemistry; Chromosomes - metabolism; Compaction; Condensation; Condensers; Condensers (liquefiers); Deoxyribonucleic acid; DNA; DNA binding proteins; Drosophila; Drosophila melanogaster - metabolism; Drosophila Proteins - chemistry; Enzymes; Gene Expression Regulation; HeLa Cells; Histone H1; Histones; Histones - chemistry; Histones - genetics; Histones - physiology; Humans; Insects; Microscopy, Atomic Force - methods; Molecular biology; Molecular Biology/Chromatin Structure; Nucleosomes; Nucleosomes - chemistry; Nucleosomes - metabolism; Phosphorylation; Protein Binding; Protein Structure, Tertiary; Proteins; Repressors; Retinoblastoma-Binding Protein 4 - chemistry; Studies; Transcription factors; Transcription Factors - chemistry; Yeast
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0007243

Although ubiquitously present in chromatin, the function of the linker histone subtypes is partly unknown and contradictory studies on their properties have been published. To explore whether the various H1 subtypes have a differential role in the organization and dynamics of chromatin we have incorporated all of the somatic human H1 subtypes into minichromosomes and compared their influence on nucleosome spacing, chromatin compaction and ATP-dependent remodeling. H1 subtypes exhibit different affinities for chromatin and different abilities to promote chromatin condensation, as studied with the Atomic Force Microscope. According to this criterion, H1 subtypes can be classified as weak condensers (H1.1 and H1.2), intermediate condensers (H1.3) and strong condensers (H1.0, H1.4, H1.5 and H1x). The variable C-terminal domain is required for nucleosome spacing by H1.4 and is likely responsible for the chromatin condensation properties of the various subtypes, as shown using chimeras between H1.4 and H1.2. In contrast to previous reports with isolated nucleosomes or linear nucleosomal arrays, linker histones at a ratio of one per nucleosome do not preclude remodeling of minichromosomes by yeast SWI/SNF or Drosophila NURF. We hypothesize that the linker histone subtypes are differential organizers of chromatin, rather than general repressors.