In vivo quantification of VCAM-1 expression in renal ischemia reperfusion injury using non-invasive magnetic resonance molecular imaging

Vascular cell adhesion molecule-1 (VCAM-1) is upregulated in ischemia reperfusion injury (IRI), persisting after restoration of blood flow. We hypothesized that microparticles of iron oxide targeting VCAM-1 (VCAM-MPIO) would depict "ischemic memory" and enable in vivo assessment of VCAM-1...

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Bibliographic Details
Published in:PloS one 2010-09, Vol.5 (9), p.e12800-e12800
Main Authors: Akhtar, Asim M, Schneider, Jurgen E, Chapman, Stephanie J, Jefferson, Andrew, Digby, Janet E, Mankia, Kulveer, Chen, Ye, McAteer, Martina A, Wood, Kathryn J, Choudhury, Robin P
Format: Article
Language:English
Subjects:
Animals
Atherosclerosis
Binding sites
Blood
Blood flow
Body weight
Brain diseases
Cardiovascular Disorders/Myocardial Infarction
Cardiovascular Disorders/Vascular Biology
Cell adhesion
Cell adhesion molecules
Cloning
Contrast media
Contrast Media - analysis
Contrast Media - metabolism
Diagnostic imaging
Disease Models, Animal
Endothelium
Ferric Compounds - analysis
Ferric Compounds - metabolism
Ferric oxide
Gene Expression
Glycoproteins
Hepatocytes
Hospitals
Humans
Image processing
Image segmentation
Immunoglobulin G
Immunoglobulins
Immunology
In vivo methods and tests
Intervention
Intravenous administration
Iron
Iron compounds
Iron oxides
Ischemia
Kidney - blood supply
Kidney - diagnostic imaging
Kidney - metabolism
Kidneys
Kupffer cells
Labeling
Liver
Macrophages
Magnetic resonance
Magnetic resonance imaging
Magnetic Resonance Imaging - methods
Male
Medicine
Mice
Mice, Inbred C57BL
Microparticles
Monocytes
NMR
Nuclear magnetic resonance
Radiography
Radiology and Medical Imaging/Magnetic Resonance Imaging
Reperfusion
Reperfusion injury
Reperfusion Injury - diagnostic imaging
Reperfusion Injury - genetics
Reperfusion Injury - metabolism
Restoration
RNA
Segmentation
Spleen
Surgery
Transcription
Vascular cell adhesion molecule 1
Vascular Cell Adhesion Molecule-1 - analysis
Vascular Cell Adhesion Molecule-1 - genetics
Vascular Cell Adhesion Molecule-1 - metabolism
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Summary:Vascular cell adhesion molecule-1 (VCAM-1) is upregulated in ischemia reperfusion injury (IRI), persisting after restoration of blood flow. We hypothesized that microparticles of iron oxide targeting VCAM-1 (VCAM-MPIO) would depict "ischemic memory" and enable in vivo assessment of VCAM-1 expression. Mice subject to unilateral, transient (30 minutes) renal ischemia and subsequent reperfusion received intravenous VCAM-MPIO (4.5 mg iron/kg body weight). Contrast agent bound rapidly (
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0012800