Ultra-deep sequencing reveals the microRNA expression pattern of the human stomach

While microRNAs (miRNAs) play important roles in tissue differentiation and in maintaining basal physiology, little is known about the miRNA expression levels in stomach tissue. Alterations in the miRNA profile can lead to cell deregulation, which can induce neoplasia. A small RNA library of stomach...

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Bibliographic Details
Published in:PloS one 2010-10, Vol.5 (10), p.e13205-e13205
Main Authors: Ribeiro-dos-Santos, Ândrea, Khayat, André S, Silva, Artur, Alencar, Dayse O, Lobato, Jessé, Luz, Larissa, Pinheiro, Daniel G, Varuzza, Leonardo, Assumpção, Monica, Assumpção, Paulo, Santos, Sidney, Zanette, Dalila L, Silva, Jr, Wilson A, Burbano, Rommel, Darnet, Sylvain
Format: Article
Language:English
Subjects:
B cells
Blood
Cancer
Deoxyribonucleic acid
Deregulation
DNA
DNA methylation
DNA sequencing
Gastric cancer
Gene expression
Gene Expression Profiling
Gene sequencing
Genetics and Genomics
Genetics and Genomics/Bioinformatics
Genetics and Genomics/Cancer Genetics
Genetics and Genomics/Comparative Genomics
Genetics and Genomics/Functional Genomics
Genetics and Genomics/Medical Genetics
Genomes
Humans
Leukemia
Metastasis
MicroRNA
MicroRNAs
MicroRNAs - genetics
miRNA
Ovarian cancer
Pancreatic cancer
Polymerase chain reaction
Reverse Transcriptase Polymerase Chain Reaction
Ribonucleic acid
RNA
Sequence Analysis, RNA
Stem cells
Stomach
Stomach - metabolism
Tissues
Umbilical cord
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Summary:While microRNAs (miRNAs) play important roles in tissue differentiation and in maintaining basal physiology, little is known about the miRNA expression levels in stomach tissue. Alterations in the miRNA profile can lead to cell deregulation, which can induce neoplasia. A small RNA library of stomach tissue was sequenced using high-throughput SOLiD sequencing technology. We obtained 261,274 quality reads with perfect matches to the human miRnome, and 42% of known miRNAs were identified. Digital Gene Expression profiling (DGE) was performed based on read abundance and showed that fifteen miRNAs were highly expressed in gastric tissue. Subsequently, the expression of these miRNAs was validated in 10 healthy individuals by RT-PCR showed a significant correlation of 83.97% (P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0013205