Altered actin centripetal retrograde flow in physically restricted immunological synapses

Antigen recognition by T cells involves large scale spatial reorganization of numerous receptor, adhesion, and costimulatory proteins within the T cell-antigen presenting cell (APC) junction. The resulting patterns can be distinctive, and are collectively known as the immunological synapse. Dynamica...

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Published in:PloS one 2010-07, Vol.5 (7), p.e11878-e11878
Main Authors: Yu, Cheng-han, Wu, Hung-jen, Kaizuka, Yoshihisa, Vale, Ronald D, Groves, Jay T
Format: Article
Language:English
Subjects:
Actin
Actins - metabolism
Algorithms
Antigen-presenting cells
Antigens
Assembly
BASIC BIOLOGICAL SCIENCES
Biophysics
Biophysics/Experimental Biophysical Methods
Biophysics/Macromolecular Assemblies and Machines
Cell adhesion & migration
Cell Biology/Cytoskeleton
Clusters
Cytoskeleton
Flow (Dynamics)
Flow pattern
Glass substrates
Humans
Immunological synapses
Immunological Synapses - metabolism
Immunology
Jurkat Cells - metabolism
Lymphocytes
Lymphocytes T
Materials science
Membranes
Microscopy
Microscopy, Confocal
Muscle proteins
Particle tracking
Proteins
Receptors, Antigen, T-Cell - metabolism
Science & Technology - Other Topics
Signal transduction
Spatial distribution
Synapses
T cell receptors
T cells
T-cell receptor
Velocity
Velocity distribution
Viscoelasticity
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cited_by cdi_FETCH-LOGICAL-c816t-49289cc15b76f6b104672c366f34eff065643cca91681a5382f3e96bb80bbc1f3
cites cdi_FETCH-LOGICAL-c816t-49289cc15b76f6b104672c366f34eff065643cca91681a5382f3e96bb80bbc1f3
container_end_page e11878
container_issue 7
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container_title PloS one
container_volume 5
creator Yu, Cheng-han
Wu, Hung-jen
Kaizuka, Yoshihisa
Vale, Ronald D
Groves, Jay T
description Antigen recognition by T cells involves large scale spatial reorganization of numerous receptor, adhesion, and costimulatory proteins within the T cell-antigen presenting cell (APC) junction. The resulting patterns can be distinctive, and are collectively known as the immunological synapse. Dynamical assembly of cytoskeletal network is believed to play an important role in driving these assembly processes. In one experimental strategy, the APC is replaced with a synthetic supported membrane. An advantage of this configuration is that solid structures patterned onto the underlying substrate can guide immunological synapse assembly into altered patterns. Here, we use mobile anti-CD3epsilon on the spatial-partitioned supported bilayer to ligate and trigger T cell receptor (TCR) in live Jurkat T cells. Simultaneous tracking of both TCR clusters and GFP-actin speckles reveals their dynamic association and individual flow patterns. Actin retrograde flow directs the inward transport of TCR clusters. Flow-based particle tracking algorithms allow us to investigate the velocity distribution of actin flow field across the whole synapse, and centripetal velocity of actin flow decreases as it moves toward the center of synapse. Localized actin flow analysis reveals that, while there is no influence on actin motion from substrate patterns directly, velocity differences of actin are observed over physically trapped TCR clusters. Actin flow regains its velocity immediately after passing through confined TCR clusters. These observations are consistent with a dynamic and dissipative coupling between TCR clusters and viscoelastic actin network.
doi_str_mv 10.1371/journal.pone.0011878
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source Publicly Available Content Database; PubMed Central
subjects Actin
Actins - metabolism
Algorithms
Antigen-presenting cells
Antigens
Assembly
BASIC BIOLOGICAL SCIENCES
Biophysics
Biophysics/Experimental Biophysical Methods
Biophysics/Macromolecular Assemblies and Machines
Cell adhesion & migration
Cell Biology/Cytoskeleton
Clusters
Cytoskeleton
Flow (Dynamics)
Flow pattern
Glass substrates
Humans
Immunological synapses
Immunological Synapses - metabolism
Immunology
Jurkat Cells - metabolism
Lymphocytes
Lymphocytes T
Materials science
Membranes
Microscopy
Microscopy, Confocal
Muscle proteins
Particle tracking
Proteins
Receptors, Antigen, T-Cell - metabolism
Science & Technology - Other Topics
Signal transduction
Spatial distribution
Synapses
T cell receptors
T cells
T-cell receptor
Velocity
Velocity distribution
Viscoelasticity
title Altered actin centripetal retrograde flow in physically restricted immunological synapses
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