Alternative splicing of sept9a and sept9b in zebrafish produces multiple mRNA transcripts expressed throughout development

Septins are involved in a number of cellular processes including cytokinesis and organization of the cytoskeleton. Alterations in human septin-9 (SEPT9) levels have been linked to multiple cancers, whereas mutations in SEPT9 cause the episodic neuropathy, hereditary neuralgic amyotrophy (HNA). Despi...

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Bibliographic Details
Published in:PloS one 2010-05, Vol.5 (5), p.e10712-e10712
Main Authors: Landsverk, Megan L, Weiser, Douglas C, Hannibal, Mark C, Kimelman, David
Format: Article
Language:English
Subjects:
Aberration
Alternative splicing
Alternative Splicing - genetics
Amino Acid Sequence
Animals
Apoptosis
Biochemistry
Brachydanio rerio
Cell Death
Conserved Sequence - genetics
Cytokinesis
Cytoskeleton
Danio rerio
Defects
Developmental Biology
Developmental Biology/Molecular Development
Developmental Biology/Morphogenesis and Cell Biology
Edema
Embryo, Nonmammalian - metabolism
Gene expression
Gene Expression Regulation, Developmental
Gene Knockdown Techniques
Genetic engineering
Genetics and Genomics/Gene Expression
Genetics and Genomics/Gene Function
Genomes
In vivo methods and tests
Laboratories
Leukemia
Messenger RNA
Molecular Sequence Data
Morphology
Motility
Mutation
Neuropathy
Pediatrics
Permeability
RNA, Messenger - genetics
RNA, Messenger - metabolism
Septin
Vertebrates
Zebrafish
Zebrafish - embryology
Zebrafish - genetics
Zebrafish Proteins - antagonists & inhibitors
Zebrafish Proteins - chemistry
Zebrafish Proteins - genetics
Zebrafish Proteins - metabolism
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Summary:Septins are involved in a number of cellular processes including cytokinesis and organization of the cytoskeleton. Alterations in human septin-9 (SEPT9) levels have been linked to multiple cancers, whereas mutations in SEPT9 cause the episodic neuropathy, hereditary neuralgic amyotrophy (HNA). Despite its important function in human health, the in vivo role of SEPT9 is unknown. Here we utilize zebrafish to study the role of SEPT9 in early development. We show that zebrafish possess two genes, sept9a and sept9b that, like humans, express multiple transcripts. Knockdown or overexpression of sept9a transcripts results in specific developmental alterations including circulation defects and aberrant epidermal development. Our work demonstrates that sept9 plays an important role in zebrafish development, and establishes zebrafish as a valuable model organism for the study of SEPT9.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0010712