Endocytosis regulates cell soma translocation and the distribution of adhesion proteins in migrating neurons

Newborn neurons migrate from their birthplace to their final location to form a properly functioning nervous system. During these movements, young neurons must attach and subsequently detach from their substrate to facilitate migration, but little is known about the mechanisms cells use to release t...

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Bibliographic Details
Published in:PloS one 2011-03, Vol.6 (3), p.e17802-e17802
Main Authors: Shieh, Jennifer C, Schaar, Bruce T, Srinivasan, Karpagam, Brodsky, Frances M, McConnell, Susan K
Format: Article
Language:English
Subjects:
Adhesion
Biology
Cell Adhesion
Cell adhesion & migration
Cell body
Cell migration
Clathrin
Clathrin - physiology
Discosoma
Dynamin
Dynamins - physiology
Dyslexia
Electroporation
Endocytosis
Extracellular matrix
Fibroblasts
Humans
Immunohistochemistry
Inhibition
Machinery
Machinery and equipment
Microscopy
Microscopy, Electron
Motility
Neonates
Nervous system
Neurons
Neurons - metabolism
Neurosciences
Proteins
Substrates
Translocation
Trends
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Summary:Newborn neurons migrate from their birthplace to their final location to form a properly functioning nervous system. During these movements, young neurons must attach and subsequently detach from their substrate to facilitate migration, but little is known about the mechanisms cells use to release their attachments. We show that the machinery for clathrin-mediated endocytosis is positioned to regulate the distribution of adhesion proteins in a subcellular region just proximal to the neuronal cell body. Inhibiting clathrin or dynamin function impedes the movement of migrating neurons both in vitro and in vivo. Inhibiting dynamin function in vitro shifts the distribution of adhesion proteins to the rear of the cell. These results suggest that endocytosis may play a critical role in regulating substrate detachment to enable cell body translocation in migrating neurons.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0017802