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Inhibition of multidrug resistance by SV40 pseudovirion delivery of an antigene peptide nucleic acid (PNA) in cultured cells
Peptide nucleic acid (PNA) is known to bind with extraordinarily high affinity and sequence-specificity to complementary nucleic acid sequences and can be used to suppress gene expression. However, effective delivery into cells is a major obstacle to the development of PNA for gene therapy applicati...
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| Published in: | PloS one 2011-03, Vol.6 (3), p.e17981-e17981 |
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| Main Authors: | , , , , , , , |
| Format: | Article |
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| container_end_page | e17981 |
| container_issue | 3 |
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| container_title | PloS one |
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| creator | Macadangdang, Benjamin Zhang, Ning Lund, Paul E Marple, Andrew H Okabe, Mitsunori Gottesman, Michael M Appella, Daniel H Kimchi-Sarfaty, Chava |
| description | Peptide nucleic acid (PNA) is known to bind with extraordinarily high affinity and sequence-specificity to complementary nucleic acid sequences and can be used to suppress gene expression. However, effective delivery into cells is a major obstacle to the development of PNA for gene therapy applications. Here, we present a novel method for the in vitro delivery of antigene PNA to cells. By using a nucleocapsid protein derived from Simian virus 40, we have been able to package PNA into pseudovirions, facilitating the delivery of the packaged PNA into cells. We demonstrate that this system can be used effectively to suppress gene expression associated with multidrug resistance in cancer cells, as shown by RT-PCR, flow cytometry, Western blotting, and cell viability under chemotherapy. The combination of PNA with the SV40-based delivery system is a method for suppressing a gene of interest that could be broadly applied to numerous targets. |
| doi_str_mv | 10.1371/journal.pone.0017981 |
| format | article |
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However, effective delivery into cells is a major obstacle to the development of PNA for gene therapy applications. Here, we present a novel method for the in vitro delivery of antigene PNA to cells. By using a nucleocapsid protein derived from Simian virus 40, we have been able to package PNA into pseudovirions, facilitating the delivery of the packaged PNA into cells. We demonstrate that this system can be used effectively to suppress gene expression associated with multidrug resistance in cancer cells, as shown by RT-PCR, flow cytometry, Western blotting, and cell viability under chemotherapy. The combination of PNA with the SV40-based delivery system is a method for suppressing a gene of interest that could be broadly applied to numerous targets.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>21445346</pmid><doi>10.1371/journal.pone.0017981</doi><oa>free_for_read</oa></addata></record> |
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| issn | 1932-6203 1932-6203 |
| language | eng |
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| source | Publicly Available Content Database; PubMed Central |
| subjects | Acids Amino acids Apoptosis ATP Binding Cassette Transporter, Sub-Family B ATP-Binding Cassette, Sub-Family B, Member 1 - physiology Biology Cancer Cells, Cultured Chemistry Chemotherapy Cytometry Deoxyribonucleic acid Diabetes DNA Drug resistance Drug Resistance, Multiple Flow cytometry Gene expression Gene sequencing Gene Silencing Gene therapy Genes Genetic engineering In vitro methods and tests Kidney diseases Laboratories Microbial drug resistance Multidrug resistance Multidrug resistant organisms Neuroblastoma Nucleic acids Nucleocapsids Peptide nucleic acids Peptide Nucleic Acids - administration & dosage Peptides Polymerase chain reaction Proteins Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics Simian virus 40 Simian virus 40 - physiology Transcription, Genetic Virion - physiology Viruses Western blotting |
| title | Inhibition of multidrug resistance by SV40 pseudovirion delivery of an antigene peptide nucleic acid (PNA) in cultured cells |
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