IL-22 production is regulated by IL-23 during Listeria monocytogenes infection but is not required for bacterial clearance or tissue protection

• Published in: PloS one 2011-02, Vol.6 (2), p.e17171-e17171
• Main Authors: Graham, Amy C, Carr, Karen D, Sieve, Amy N, Indramohan, Mohanalaxmi, Break, Timothy J, Berg, Rance E
• Format: Article
• Language: English
• Subjects: Adaptive immunity; Animals; Antiinfectives and antibacterials; Antimicrobial peptides; Apoptosis; Bacteria; Biology; Citrobacter; Coinfection - immunology; Coinfection - metabolism; Coinfection - pathology; Contaminants; Cytokines; Dairy products; Damage prevention; Disseminated infection; Female; Food contamination; Health aspects; Immune clearance; Immune response; Immunology; In vivo methods and tests; Infection; Infections; Inflammatory bowel disease; Ingestion; Interleukin 17; Interleukin 22; Interleukin 23; Interleukin-23 - metabolism; Interleukins; Interleukins - biosynthesis; Klebsiella pneumoniae; Laboratory animals; Leukocytes (neutrophilic); Listeria; Listeria monocytogenes; Listeria monocytogenes - pathogenicity; Listeriosis - immunology; Listeriosis - metabolism; Listeriosis - pathology; Liver; Lymphocytes; Male; Medicine; Meningitis; Mice; Microorganisms; Miscarriage; Molecular biology; Mucosa; Mucous Membrane - immunology; Mucous Membrane - metabolism; Mucous Membrane - microbiology; Peptides; Rodents; Science; Secondary infection; Septicemia; Spleen; Studies; Survival Analysis; Tissues; Toxoplasma gondii
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0017171

Listeria monocytogenes (LM) is a gram-positive bacterium that is a common contaminant of processed meats and dairy products. In humans, ingestion of LM can result in intracellular infection of the spleen and liver, which can ultimately lead to septicemia, meningitis, and spontaneous abortion. Interleukin (IL)-23 is a cytokine that regulates innate and adaptive immune responses by inducing the production of IL-17A, IL-17F, and IL-22. We have recently demonstrated that the IL-23/IL-17 axis is required for optimal recruitment of neutrophils to the liver, but not the spleen, during LM infection. Furthermore, these cytokines are required for the clearance of LM during systemic infection. In other infectious models, IL-22 induces the secretion of anti-microbial peptides and protects tissues from damage by preventing apoptosis. However, the role of IL-22 has not been thoroughly investigated during LM infection. In the present study, we show that LM induces the production of IL-22 in vivo. Interestingly, IL-23 is required for the production of IL-22 during primary, but not secondary, LM infection. Our findings suggest that IL-22 is not required for clearance of LM during primary or secondary infection, using both systemic and mucosal models of infection. IL-22 is also not required for the protection of LM infected spleens and livers from organ damage. Collectively, these data indicate that IL-22 produced during LM infection must play a role other than clearance of LM or protection of tissues from pathogen- or immune-mediated damage.