Profiling the dead : generating microsatellite data from fossil bones of extinct megafauna - protocols, problems, and prospects

Presents the first set of microsatellite markers developed exclusively for an extinct taxon with low copy number (LCN) DNA - the extinct New Zealand moa (Aves: Dinornithiformes). Demonstrates the viability of the primers and the protocols used, by compiling a full Dinornis microsatellite dataset rep...

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Bibliographic Details
Published in:PloS one 2011-01, Vol.6 (1), p.e16670-e16670
Main Author: Allentoft, Morten E
Format: Article
Language:English
Subjects:
Agkistrodon contortrix
Analysis
Animals
Animals, Fossil
Aves
Biology
Biotechnology
Birds
Birds, Fossil
Bone and Bones
Bones
Copy number
Data processing
Deoxyribonucleic acid
Dinornis
DNA
DNA Primers
DNA sequencing
DNA, Fossil
DNA, Mitochondrial - isolation & purification
Egg Shell
Egg shells
Endangered & extinct species
Extinct birds
Extinction
Extinction, Biological
Fossils
Gene sequencing
Genetic aspects
Genetic diversity
Genetic markers
Genetics
Genomes
Identification
Laboratories
Leiopelma hochstetteri
Loci
Markers
Megafauna
Microsatellite Repeats - genetics
Microsatellites
Microsatellites (Genetics)
Mitochondrial DNA
Moa
Museums
Natural history
Next-generation sequencing
Palaeognathae
Polymerase chain reaction
Polymorphism
Population genetics
Preservation
Primers
Research Design
Single nucleotide polymorphisms
Single-nucleotide polymorphism
Studies
Substrates
Technology
Viability
Wildlife conservation
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Description
Summary:Presents the first set of microsatellite markers developed exclusively for an extinct taxon with low copy number (LCN) DNA - the extinct New Zealand moa (Aves: Dinornithiformes). Demonstrates the viability of the primers and the protocols used, by compiling a full Dinornis microsatellite dataset representing fossils of c. 600–5000 years of age. Source: National Library of New Zealand Te Puna Matauranga o Aotearoa, licensed by the Department of Internal Affairs for re-use under the Creative Commons Attribution 3.0 New Zealand Licence.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0016670