Fission of tubular endosomes triggers endosomal acidification and movement

The early endosome acts as a sorting station for internalized molecules destined for recycling or degradation. While recycled molecules are sorted and delivered to tubular endosomes, residual compartments containing molecules to be degraded undergo "maturation" before final degradation in...

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Bibliographic Details
Published in:PloS one 2011-05, Vol.6 (5), p.e19764-e19764
Main Authors: Mesaki, Kumi, Tanabe, Kenji, Obayashi, Masanori, Oe, Natsuko, Takei, Kohji
Format: Article
Language:English
Subjects:
Acidification
Acids - metabolism
Biological Transport - drug effects
Biology
Cell Nucleus - drug effects
Cell Nucleus - metabolism
Compartments
Degradation
Dentistry
Depolymerization
Dynamin
Endocytosis
Endocytosis - drug effects
Endosomes
Endosomes - drug effects
Endosomes - metabolism
Endosomes - ultrastructure
Epidermal growth factor
Epidermal Growth Factor - pharmacology
Fission
HeLa Cells
Humans
Hydrazones - pharmacology
Localization
Maturation
Medical research
Medicine
Microtubules
Microtubules - drug effects
Microtubules - metabolism
Microtubules - ultrastructure
Movement - drug effects
Neurosciences
Pharmaceutical sciences
Protein turnover
Proteins
Recycling
Studies
Transferrin - metabolism
Trends
Tubules
University graduates
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Summary:The early endosome acts as a sorting station for internalized molecules destined for recycling or degradation. While recycled molecules are sorted and delivered to tubular endosomes, residual compartments containing molecules to be degraded undergo "maturation" before final degradation in the lysosome. This maturation involves acidification, microtubule-dependent motility, and perinuclear localization. It is currently unknown how sorting and the processes of maturation cooperate with each other. Here, we show that fission of a tubular endosome triggers the maturation of the residual endosome, leading to degradation. Use of the dynamin inhibitor dynasore to block tubular endosome fission inhibited acidification, endosomal motility along microtubules, perinuclear localization, and degradation. However, tubular endosome fission was not affected by inhibiting endosomal acidification or by depolymerizing the microtubules. These results demonstrate that the fission of recycling tubules is the first important step in endosomal maturation and degradation in the lysosome. We believe this to be the first evidence of a cascade from sorting to degradation.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0019764