Individual differences in AMY1 gene copy number, salivary α-amylase levels, and the perception of oral starch

• Published in: PloS one 2010-10, Vol.5 (10), p.e13352-e13352
• Main Authors: Mandel, Abigail L, Peyrot des Gachons, Catherine, Plank, Kimberly L, Alarcon, Suzanne, Breslin, Paul A S
• Format: Article
• Language: English
• Subjects: alpha-Amylases - genetics; alpha-Amylases - metabolism; AMY1 gene; Amylases; Copy number; Diet; Dietary intake; Digestion; Enzymatic activity; Enzymes; Evolutionary Biology/Human Evolution; Fat substitutes; Food; Gene Dosage; Genetic diversity; Genetic engineering; Genetics and Genomics; Humans; Maltose; Maltotriose; Mechanical properties; Milk; Mouth - metabolism; Neuroscience/Psychology; Neuroscience/Sensory Systems; Nutrition; Nutritional status; Oils & fats; Oligosaccharides; Perception; Perceptions; Physical properties; Proteins; Rheology; Saliva; Saliva - enzymology; Senses; Starch; Starch - metabolism; Variation; Viscosity; α-Amylase
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0013352

The digestion of dietary starch in humans is initiated by salivary α-amylase, an endo-enzyme that hydrolyzes starch into maltose, maltotriose and larger oligosaccharides. Salivary amylase accounts for 40 to 50% of protein in human saliva and rapidly alters the physical properties of starch. Importantly, the quantity and enzymatic activity of salivary amylase show significant individual variation. However, linking variation in salivary amylase levels with the oral perception of starch has proven difficult. Furthermore, the relationship between copy number variations (CNVs) in the AMY1 gene, which influence salivary amylase levels, and starch viscosity perception has not been explored. Here we demonstrate that saliva containing high levels of amylase has sufficient activity to rapidly hydrolyze a viscous starch solution in vitro. Furthermore, we show with time-intensity ratings, which track the digestion of starch during oral manipulation, that individuals with high amylase levels report faster and more significant decreases in perceived starch viscosity than people with low salivary amylase levels. Finally, we demonstrate that AMY1 CNVs predict an individual's amount and activity of salivary amylase and thereby, ultimately determine their perceived rate of oral starch viscosity thinning. By linking genetic variation and its consequent salivary enzymatic differences to the perceptual sequellae of these variations, we show that AMY1 copy number relates to salivary amylase concentration and enzymatic activity level, which, in turn, account for individual variation in the oral perception of starch viscosity. The profound individual differences in salivary amylase levels and salivary activity may contribute significantly to individual differences in dietary starch intake and, consequently, to overall nutritional status.