Clathrin couture: fashioning distinctive membrane coats at the cell surface

  First identified to be cargo-selective transport carriers during yolk uptake and storage within oocytes of blood-fed mosquitoes [2], clathrin-coated vesicles are now known to support many vital cellular processes, ranging from nutrient uptake, cellular locomotion, and transcriptional regulation an...

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Bibliographic Details
Published in:PLoS biology 2009-09, Vol.7 (9), p.e1000192-e1000192
Main Author: Traub, Linton M
Format: Article
Language:English
Subjects:
Animals
Cell Biology/Membranes and Sorting
Cellular biology
Clathrin
Clathrin - metabolism
Coated Pits, Cell-Membrane - physiology
Coated Pits, Cell-Membrane - ultrastructure
Endocytosis
Endocytosis - physiology
HeLa Cells
Humans
Membranes (Biology)
Models, Biological
Observations
Physiological aspects
Plasma
Primer
Proteins
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Summary:  First identified to be cargo-selective transport carriers during yolk uptake and storage within oocytes of blood-fed mosquitoes [2], clathrin-coated vesicles are now known to support many vital cellular processes, ranging from nutrient uptake, cellular locomotion, and transcriptional regulation and proliferation to complex developmental morphogenetic events. Perturbing PtdIns(4,5)P2 production in cultured cells leads to an almost immediate dissolution of preexisting clathrin-coated structures at the cell surface [8],[10],[11]. Because transmission electron microscope (EM) images typically reveal isolated, invaginating coated buds all along the cell surface, and clathrin immunolabeling often shows a profusion of small, separated puncta apparently randomly scattered over the surface membrane (Figure 2), it seems reasonable to suspect that clathrin-coated vesicles might form de novo for each internalization cycle.
ISSN:1545-7885
1544-9173
1545-7885
DOI:10.1371/journal.pbio.1000192