A systematic molecular pathology study of a laboratory confirmed H5N1 human case

• Published in: PloS one 2010-10, Vol.5 (10), p.e13315-e13315
• Main Authors: Gao, Rongbao, Dong, Libo, Dong, Jie, Wen, Leying, Zhang, Ye, Yu, Hongjie, Feng, Zijian, Chen, Minmei, Tan, Yi, Mo, Zhaojun, Liu, Haiyan, Fan, Yunyan, Li, Kunxiong, Li, Chris Ka-Fai, Li, Dexin, Yang, Weizhong, Shu, Yuelong
• Format: Article
• Language: English
• Subjects: Adult; Autopsies; Autopsy; Avian flu; Avian influenza; Avian influenza virus; Avian influenza viruses; Brain; Brain stem; Bronchus; Cerebellum; Cerebral cortex; Chemokines; Colon; Cortex; Cytokines; Development and progression; Fatal Outcome; Fowl plague; Genetic engineering; Health aspects; Hemagglutinins; Hippocampus; Humans; Ileum; Immune system; Immunohistochemistry; Infection; Infections; Influenza A Virus, H5N1 Subtype - genetics; Influenza A Virus, H5N1 Subtype - isolation & purification; Influenza, Human - genetics; Influenza, Human - pathology; Influenza, Human - virology; Lesions; Liver; Lung; Lungs; Lymph nodes; Male; Medical research; Organs; Pandemics; Pathogenesis; Pathology/Immunology; Pathology/Molecular Pathology; Polymerase chain reaction; Real time; Rectum; Replication; Respiratory system; Reverse Transcriptase Polymerase Chain Reaction; Spleen; Stains; Tissues; Trachea; Tumor necrosis factor-TNF; Ureter; Viral Load; Virology/Emerging Viral Diseases; Virus diseases; Viruses
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0013315

Autopsy studies have shown that human highly pathogenic avian influenza virus (H5N1) can infect multiple human organs other than just the lungs, and that possible causes of organ damage are either viral replication and/or dysregulation of cytokines and chemokines. Uncertainty still exists, partly because of the limited number of cases analysed. In this study, a full autopsy including 5 organ systems was conducted on a confirmed H5N1 human fatal case (male, 42 years old) within 18 hours of death. In addition to the respiratory system (lungs, bronchus and trachea), virus was isolated from cerebral cortex, cerebral medullary substance, cerebellum, brain stem, hippocampus ileum, colon, rectum, ureter, aortopulmonary vessel and lymph-node. Real time RT-PCR evidence showed that matrix and hemagglutinin genes were positive in liver and spleen in addition to positive tissues with virus isolation. Immunohistochemistry and in-situ hybridization stains showed accordant evidence of viral infection with real time RT-PCR except bronchus. Quantitative RT-PCR suggested that a high viral load was associated with increased host responses, though the viral load was significantly different in various organs. Cells of the immunologic system could also be a target for virus infection. Overall, the pathogenesis of HPAI H5N1 virus was associated both with virus replication and with immunopathologic lesions. In addition, immune cells cannot be excluded from playing a role in dissemination of the virus in vivo.