Antitumor activity of Noscapine in combination with Doxorubicin in triple negative breast cancer

• Published in: PloS one 2011-03, Vol.6 (3), p.e17733
• Main Authors: Chougule, Mahavir B, Patel, Apurva R, Jackson, Tanise, Singh, Mandip
• Format: Article
• Language: English
• Subjects: Acids; Angiogenesis; Angiogenesis Inducing Agents - metabolism; Animal tissues; Animals; Anthracyclines; Anticancer properties; Antineoplastic Agents - pharmacology; Antineoplastic Agents - therapeutic use; Antineoplastic Combined Chemotherapy Protocols - pharmacology; Antineoplastic Combined Chemotherapy Protocols - therapeutic use; Antitumor activity; Apoptosis; Apoptosis - drug effects; Apoptosis Regulatory Proteins - metabolism; Biology; Breast cancer; Breast Neoplasms - blood supply; Breast Neoplasms - drug therapy; Breast Neoplasms - enzymology; Breast Neoplasms - pathology; Cancer; Cancer therapies; Cancer treatment; Caspase 3 - metabolism; Cell cycle; Cell Line, Tumor; Cell Proliferation - drug effects; Cell survival; Cell Survival - drug effects; Chemotherapy; Colorectal cancer; Cytokines; Cytotoxicity; Deactivation; Development and progression; DNA Fragmentation - drug effects; Doxorubicin; Doxorubicin - pharmacology; Doxorubicin - therapeutic use; Drug dosages; Drug Synergism; Female; Gastric cancer; Humans; Immunohistochemistry; Inactivation; Laboratory animals; Lung cancer; Medicine; Metastasis; Mice; Neovascularization, Pathologic - drug therapy; Neovascularization, Pathologic - pathology; NF-kappa B - metabolism; NF-κB protein; Noscapine - pharmacology; Noscapine - therapeutic use; Pharmacy; Prostate cancer; Proteins; Staining; Stomach cancer; Tumorigenesis; Tumors; Vascular endothelial growth factor; Xenograft Model Antitumor Assays; Xenografts
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0017733

The aim of this study was to investigate the anticancer activity and mechanism of action of Noscapine alone and in combination with Doxorubicin against triple negative breast cancer (TNBC). TNBC cells were pretreated with Noscapine or Doxorubicin or combination and combination index values were calculated using isobolographic method. Apoptosis was assessed by TUNEL staining. Female athymic Nu/nu mice were xenografted with MDA-MB-231 cells and the efficacy of Noscapine, Doxorubicin and combination was determined. Protein expression, immunohistochemical staining were evaluated in harvested tumor tissues. Noscapine inhibited growth of MDA-MB-231 and MDA-MB-468 cells with the IC(50) values of 36.16±3.76 and 42.7±4.3 µM respectively. The CI values (