Effects of altered maternal folic acid, vitamin B12 and docosahexaenoic acid on placental global DNA methylation patterns in Wistar rats

Potential adverse effects of excess maternal folic acid supplementation on a vegetarian population deficient in vitamin B(12) are poorly understood. We have previously shown in a rat model that maternal folic acid supplementation at marginal protein levels reduces brain omega-3 fatty acid levels in...

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Bibliographic Details
Published in:PloS one 2011-03, Vol.6 (3), p.e17706-e17706
Main Authors: Kulkarni, Asmita, Dangat, Kamini, Kale, Anvita, Sable, Pratiksha, Chavan-Gautam, Preeti, Joshi, Sadhana
Format: Article
Language:English
Subjects:
Animals
Biology
Biomarkers
Brain
Brain research
Cyanocobalamin
Defects
Deoxyribonucleic acid
Diet
Dietary supplements
DNA
DNA Methylation
Docosahexaenoic acid
Docosahexaenoic Acids - blood
Drug dosages
Epigenetics
Fatty acids
Fatty Acids - blood
Feeding Behavior
Female
Folic acid
Folic Acid - blood
Gene expression
Genomes
Homocysteine
Homocysteine - blood
Hypertension
Insulin resistance
Laboratory animals
Ligands
Medicine
Metabolism
Nutrient deficiency
Nutrition research
Offspring
Omega-3 fatty acids
Placenta
Placenta - metabolism
Preeclampsia
Pregnancy
Rats
Reproduction
Rodents
Studies
Vegetarianism
Vitamin B
Vitamin B 12 - blood
Vitamin B12
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Summary:Potential adverse effects of excess maternal folic acid supplementation on a vegetarian population deficient in vitamin B(12) are poorly understood. We have previously shown in a rat model that maternal folic acid supplementation at marginal protein levels reduces brain omega-3 fatty acid levels in the adult offspring. We have also reported that reduced docosahexaenoic acid (DHA) levels may result in diversion of methyl groups towards DNA in the one carbon metabolic pathway ultimately resulting in DNA methylation. This study was designed to examine the effect of normal and excess folic acid in the absence and presence of vitamin B(12) deficiency on global methylation patterns in the placenta. Further, the effect of maternal omega 3 fatty acid supplementation on the above vitamin B(12) deficient diets was also examined. Our results suggest maternal folic acid supplementation in the absence of vitamin B(12) lowers plasma and placental DHA levels (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0017706