The Candida albicans-specific gene EED1 encodes a key regulator of hyphal extension

The extension of germ tubes into elongated hyphae by Candida albicans is essential for damage of host cells. The C. albicans-specific gene EED1 plays a crucial role in this extension and maintenance of filamentous growth. eed1Δ cells failed to extend germ tubes into long filaments and switched back...

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Bibliographic Details
Published in:PloS one 2011-04, Vol.6 (4), p.e18394-e18394
Main Authors: Martin, Ronny, Moran, Gary P, Jacobsen, Ilse D, Heyken, Antje, Domey, Jenny, Sullivan, Derek J, Kurzai, Oliver, Hube, Bernhard
Format: Article
Language:English
Subjects:
Baking yeast
Biology
Candida albicans
Candida albicans - genetics
Candida albicans - growth & development
Candida albicans - metabolism
Damage detection
E coli
Elongation
Filamentation
Filaments
Fungal Proteins - genetics
Fungal Proteins - metabolism
Gene expression
Gene Expression Regulation, Fungal - genetics
Gene Expression Regulation, Fungal - physiology
Gene regulation
Genes
Germ tubes
Health aspects
Hyphae
Hyphae - genetics
Hyphae - growth & development
Hyphae - metabolism
Infection
Infections
Kinases
Mathematical models
Medicine
Morphology
Mutation
Natural products
Pathogens
Physiology
Plastics
Proteins
Saccharomyces cerevisiae
Signal transduction
Tissues
Transcription (Genetics)
Tubes
Yeast
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Summary:The extension of germ tubes into elongated hyphae by Candida albicans is essential for damage of host cells. The C. albicans-specific gene EED1 plays a crucial role in this extension and maintenance of filamentous growth. eed1Δ cells failed to extend germ tubes into long filaments and switched back to yeast growth after 3 h of incubation during growth on plastic surfaces. Expression of EED1 is regulated by the transcription factor Efg1 and ectopic overexpression of EED1 restored filamentation in efg1Δ. Transcriptional profiling of eed1Δ during infection of oral tissue revealed down-regulation of hyphal associated genes including UME6, encoding another key transcriptional factor. Ectopic overexpression of EED1 or UME6 rescued filamentation and damage potential in eed1Δ. Transcriptional profiling during overexpression of UME6 identified subsets of genes regulated by Eed1 or Ume6. These data suggest that Eed1 and Ume6 act in a pathway regulating maintenance of hyphal growth thereby repressing hyphal-to-yeast transition and permitting dissemination of C. albicans within epithelial tissues.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0018394