The histone H2A variant macroH2A1 does not localize to the centrosome

MacroH2A1 is a histone H2A variant which contains a large non-histone C-terminal region of largely unknown function. Within this region is a macro domain which can bind ADP-ribose and related molecules. Most studies of macroH2A1 focus on the involvement of this variant in transcriptional repression....

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Bibliographic Details
Published in:PloS one 2011-02, Vol.6 (2), p.e17262
Main Authors: Friedman, Nathalie, Barzily-Rokni, Michal, Isaac, Sara, Eden, Amir
Format: Article
Language:English
Subjects:
Adenosine diphosphate
Animals
Antibodies
Antigen-antibody reactions
Biology
Cell cycle
Cells, Cultured
Centrosome - metabolism
Chromatin - metabolism
Chromosomes
Cloning
Cross-reactivity
Deoxyribonucleic acid
Developmental biology
DNA
Embryo cells
Embryogenesis
Embryonic development
Embryonic stem cells
Embryos
Gene expression
Gene Knockdown Techniques
Gene silencing
Genetic Variation - physiology
Green Fluorescent Proteins - metabolism
Histone H2A
Histones - antagonists & inhibitors
Histones - genetics
Histones - metabolism
Humans
Life sciences
Localization
Lung cancer
Mice
Mutant Proteins - metabolism
Protein Transport - genetics
Protein Transport - physiology
Proteins
Recombinant Fusion Proteins - metabolism
Ribose
Somatic cells
Stem cell transplantation
Stem cells
Tissue Distribution
Transfection
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Summary:MacroH2A1 is a histone H2A variant which contains a large non-histone C-terminal region of largely unknown function. Within this region is a macro domain which can bind ADP-ribose and related molecules. Most studies of macroH2A1 focus on the involvement of this variant in transcriptional repression. Studies in mouse embryos and in embryonic stem cells suggested that during early development macroH2A can be found at the centrosome. Centrosomal localization of macroH2A was later reported in somatic cells. Here we provide data showing that macroH2A1 does not localize to the centrosome and that the centrosomal signal observed with antibodies directed against the macroH2A1 non-histone region may be the result of antibody cross-reactivity.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0017262