E. coli Nissle 1917 Affects Salmonella adhesion to porcine intestinal epithelial cells

• Published in: PloS one 2011-02, Vol.6 (2), p.e14712-e14712
• Main Authors: Schierack, Peter, Kleta, Sylvia, Tedin, Karsten, Babila, Julius Tachu, Oswald, Sibylle, Oelschlaeger, Tobias A, Hiemann, Rico, Paetzold, Susanne, Wieler, Lothar H
• Format: Article
• Language: English
• Subjects: Adhesion; Analysis; Animals; Antibiosis - physiology; Bacteria; Bacterial Adhesion - physiology; Cell culture; Cells, Cultured; E coli; Environmental regulations; Epithelial cells; Epithelial Cells - metabolism; Epithelial Cells - microbiology; Epithelial Cells - pathology; Escherichia coli; Escherichia coli - growth & development; Escherichia coli - physiology; Escherichia coli Infections - complications; Escherichia coli Infections - microbiology; Escherichia coli Infections - veterinary; Gene expression; Gene Expression Regulation, Bacterial; Gene regulation; Genes; Genetic aspects; Growth rate; Health aspects; Infection; Infections; Infectious Diseases/Bacterial Infections; Infectious Diseases/Gastrointestinal Infections; Intestinal Mucosa - metabolism; Intestinal Mucosa - microbiology; Intestinal Mucosa - pathology; Intestine; Intracellular; Lactobacillus acidophilus; Lactobacillus casei; Microbiology/Cellular Microbiology and Pathogenesis; Microbiology/Medical Microbiology; Molecular modelling; Mutants; Pathogens; Pili; Probiotics; Regulation; Salmonella; Salmonella Infections, Animal - complications; Salmonella Infections, Animal - microbiology; Salmonella Typhimurium; Salmonella typhimurium - genetics; Salmonella typhimurium - growth & development; Salmonella typhimurium - physiology; Species Specificity; Swine - microbiology; Swine - physiology; Swine Diseases - microbiology; Swine Diseases - pathology; Virulence; Virulence (Microbiology)
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0014712

The probiotic Escherichia coli strain Nissle 1917 (EcN) has been shown to interfere in a human in vitro model with the invasion of several bacterial pathogens into epithelial cells, but the underlying molecular mechanisms are not known. In this study, we investigated the inhibitory effects of EcN on Salmonella Typhimurium invasion of porcine intestinal epithelial cells, focusing on EcN effects on the various stages of Salmonella infection including intracellular and extracellular Salmonella growth rates, virulence gene regulation, and adhesion. We show that EcN affects the initial Salmonella invasion steps by modulating Salmonella virulence gene regulation and Salmonella SiiE-mediated adhesion, but not extra- and intracellular Salmonella growth. However, the inhibitory activity of EcN against Salmonella invasion always correlated with EcN adhesion capacities. EcN mutants defective in the expression of F1C fimbriae and flagellae were less adherent and less inhibitory toward Salmonella invasion. Another E. coli strain expressing F1C fimbriae was also adherent to IPEC-J2 cells, and was similarly inhibitory against Salmonella invasion like EcN. We propose that EcN affects Salmonella adhesion through secretory components. This mechanism appears to be common to many E. coli strains, with strong adherence being a prerequisite for an effective reduction of SiiE-mediated Salmonella adhesion.