A soluble acetylcholinesterase provides chemical defense against xenobiotics in the pinewood nematode

The pinewood nematode genome encodes at least three distinct acetylcholinesterases (AChEs). To understand physiological roles of the three pinewood nematode AChEs (BxACE-1, BxACE-2, and BxACE-3), BxACE-3 in particular, their tissue distribution and inhibition profiles were investigated. Immunohistoc...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2011-04, Vol.6 (4), p.e19063-e19063
Main Authors: Kang, Jae Soon, Lee, Dae-Weon, Koh, Young Ho, Lee, Si Hyeock
Format: Article
Language:English
Subjects:
Acetylcholinesterase
Acetylcholinesterase - genetics
Acetylcholinesterase - metabolism
Agricultural biotechnology
Agriculture
Animals
Antigens
Biology
Biosynthesis
Blotting, Western
Bursaphelenchus xylophilus
Chemical defense
Cholinesterase
Cholinesterase inhibitors
Electrophoresis, Polyacrylamide Gel
Enzymes
Evolution
Food
Gene Knockdown Techniques
Genomes
Immunohistochemistry
Inhibition
Life sciences
Nematoda - drug effects
Nematoda - enzymology
Nematodes
Nervous system
Oils & fats
Pharmacy
Physiology
Ribonucleic acid
RNA
RNA Interference
RNA-mediated interference
Solubility
Synaptic transmission
Tissues
Toxicity
Trends
Xenobiotics
Xenobiotics - pharmacology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The pinewood nematode genome encodes at least three distinct acetylcholinesterases (AChEs). To understand physiological roles of the three pinewood nematode AChEs (BxACE-1, BxACE-2, and BxACE-3), BxACE-3 in particular, their tissue distribution and inhibition profiles were investigated. Immunohistochemistry revealed that BxACE-1 and BxACE-2 were distributed in neuronal tissues. In contrast, BxACE-3 was detected from some specific tissues and extracted without the aid of detergent, suggesting its soluble nature unlike BxACE-1 and BxACE-2. When present together, BxAChE3 significantly reduced the inhibition of BxACE-1 and BxACE-2 by cholinesterase inhibitors. Knockdown of BxACE-3 by RNA interference significantly increased the toxicity of three nematicidal compounds, supporting the protective role of BxACE-3 against chemicals. In summary, BxACE-3 appears to have a non-neuronal function of chemical defense whereas both BxACE-1 and BxACE-2 have classical neuronal function of synaptic transmission.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0019063