Regulation of zebrafish hatching by tetraspanin cd63

Tetraspanins cause the clustering of membrane proteins into a level of organisation essential for cellular function. Given the importance and complicated nature of this mechanism, we attempted a novel approach to identify the function of a single component in a biologically relevant context. A morph...

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Bibliographic Details
Published in:PloS one 2011-05, Vol.6 (5), p.e19683-e19683
Main Authors: Trikić, Michael Z, Monk, Pete, Roehl, Henry, Partridge, Lynda J
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Biology
CD63 antigen
Cell adhesion & migration
Chorion
Clustering
Danio rerio
Dendritic cells
Embryo, Nonmammalian - cytology
Embryo, Nonmammalian - physiology
Embryonic development
Embryos
Enzymes
Gene expression
Hatching
Immunology
Intracellular
Intracellular levels
Kinases
Melanoma
Membrane proteins
Models, Animal
Molecular biology
Molecular Sequence Data
Proteases
Protein transport
Proteins
Proteolysis
Proteolytic enzymes
Tetraspanin 30 - metabolism
Zebrafish
Zebrafish - physiology
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Summary:Tetraspanins cause the clustering of membrane proteins into a level of organisation essential for cellular function. Given the importance and complicated nature of this mechanism, we attempted a novel approach to identify the function of a single component in a biologically relevant context. A morpholino knockdown strategy was used to investigate the role of cd63, a membrane protein associated with intracellular transport and a melanoma marker, in embryonic zebrafish. By using three separate morpholinos targeting cd63, we were able to identify a specific phenotype. Strikingly, morphant fish failed to hatch due to the lack of secreted proteolytic enzymes required for chorion-softening. The morphology of the hatching gland at both the cellular and intracellular levels was disorganised, suggesting a role for cd63 in the functioning of this organ. This work identifies a specific role for cd63 in the zebrafish embryo and provides evidence for the suitability of zebrafish as a model system for the investigation of tetraspanin enriched microdomains.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0019683