Secretor genotype (FUT2 gene) is strongly associated with the composition of Bifidobacteria in the human intestine

Intestinal microbiota plays an important role in human health, and its composition is determined by several factors, such as diet and host genotype. However, thus far it has remained unknown which host genes are determinants for the microbiota composition. We studied the diversity and abundance of d...

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Bibliographic Details
Published in:PloS one 2011-05, Vol.6 (5), p.e20113-e20113
Main Authors: Wacklin, Pirjo, Mäkivuokko, Harri, Alakulppi, Noora, Nikkilä, Janne, Tenkanen, Heli, Räbinä, Jarkko, Partanen, Jukka, Aranko, Kari, Mättö, Jaana
Format: Article
Language:English
Subjects:
Abundance
Analysis
Antigenic determinants
Antigens
Bacteria
Bifidobacterium - isolation & purification
Bioinformatics
Biology
Blood
Blood groups
Deoxyribonucleic acid
Determinants
DNA
Electrophoresis, Polyacrylamide Gel
Enzymes
Epitopes
Fucosyltransferases - genetics
FUT2 gene
Galactoside 2-a-L-fucosyltransferase
Galactoside 2-alpha-L-fucosyltransferase
Genes
Genetic aspects
Genetic research
Genotype
Genotypes
Glycan
Humans
Intestinal microflora
Intestine
Intestines - microbiology
Irritable bowel syndrome
Microbiota (Symbiotic organisms)
Mucosa
Mucus
Mutation
Nonsense mutation
Polymerase Chain Reaction
Principal components analysis
Secretions
Studies
Twins
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Summary:Intestinal microbiota plays an important role in human health, and its composition is determined by several factors, such as diet and host genotype. However, thus far it has remained unknown which host genes are determinants for the microbiota composition. We studied the diversity and abundance of dominant bacteria and bifidobacteria from the faecal samples of 71 healthy individuals. In this cohort, 14 were non-secretor individuals and the remainders were secretors. The secretor status is defined by the expression of the ABH and Lewis histo-blood group antigens in the intestinal mucus and other secretions. It is determined by fucosyltransferase 2 enzyme, encoded by the FUT2 gene. Non-functional enzyme resulting from a nonsense mutation in the FUT2 gene leads to the non-secretor phenotype. PCR-DGGE and qPCR methods were applied for the intestinal microbiota analysis. Principal component analysis of bifidobacterial DGGE profiles showed that the samples of non-secretor individuals formed a separate cluster within the secretor samples. Moreover, bifidobacterial diversity (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0020113