A blossoming field of research: how florigen is transported to create flowers

By linking FTIP1 to green fluorescent protein (GFP), the scientists found FTIP1 was located in the endoplasmic reticulum (ER), which helps transport proteins in cells. To see if FTIP1 and FT interact, the researchers used in situ proximity ligation assays, which target a potentially interacting pair...

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Bibliographic Details
Published in:PLoS biology 2012-04, Vol.10 (4), p.e1001311-e1001311
Main Author: Choi, Charles Q
Format: Article
Language:English
Subjects:
Arabidopsis - physiology
Arabidopsis Proteins - metabolism
Arabidopsis Proteins - physiology
Biology
Florigen - metabolism
Flowers & plants
Genes
Inflorescence - physiology
Medical research
Membrane Proteins - physiology
Microscopy
Mysteries
Physiological aspects
Plant hormones
Plants, Flowering of
Proteins
R&D
Research & development
Yeast
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Summary:By linking FTIP1 to green fluorescent protein (GFP), the scientists found FTIP1 was located in the endoplasmic reticulum (ER), which helps transport proteins in cells. To see if FTIP1 and FT interact, the researchers used in situ proximity ligation assays, which target a potentially interacting pair of proteins with pairs of antibodies that generate detectable circular DNA strands as markers if the interaction takes place.
ISSN:1545-7885
1544-9173
1545-7885
DOI:10.1371/journal.pbio.1001311