Wdr74 is required for blastocyst formation in the mouse

Preimplantation is a dynamic developmental period during which a combination of maternal and zygotic factors program the early embryo resulting in lineage specification and implantation. A reverse genetic RNAi screen in mouse embryos identified the WD Repeat Domain 74 gene (Wdr74) as being required...

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Bibliographic Details
Published in:PloS one 2011-07, Vol.6 (7), p.e22516
Main Authors: Maserati, Marc, Walentuk, Melanie, Dai, Xiangpeng, Holston, Olivia, Adams, Danielle, Mager, Jesse
Format: Article
Language:English
Subjects:
Animal sciences
Animals
Apoptosis
Apoptosis - genetics
Biology
Blastocyst - cytology
Blastocyst - metabolism
Blastocysts
Carrier Proteins
Cell Differentiation - genetics
Cell division
Deoxyribonucleic acid
Developmental stages
DNA
DNA repair
DNA-directed RNA polymerase
Embryonic development
Embryos
Epigenetics
Female
Gene expression
Gene Expression Regulation, Developmental
Genes
Genomes
Implantation
Injections
Male
Mammals
Mice
Mutation
Physics
Proteins
Proteins - genetics
Proteins - metabolism
Ribonucleic acid
RNA
RNA polymerase
RNA processing
RNA, Double-Stranded - genetics
RNA-Binding Proteins
RNA-mediated interference
Transcription
Transcription (Genetics)
Trophectoderm
Tumor Suppressor Protein p53 - genetics
Tumor Suppressor Protein p53 - metabolism
Zoology
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Summary:Preimplantation is a dynamic developmental period during which a combination of maternal and zygotic factors program the early embryo resulting in lineage specification and implantation. A reverse genetic RNAi screen in mouse embryos identified the WD Repeat Domain 74 gene (Wdr74) as being required for these critical first steps of mammalian development. Knockdown of Wdr74 results in embryos that develop normally until the morula stage but fail to form blastocysts or properly specify the inner cell mass and trophectoderm. In Wdr74-deficient embryos, we find activated Trp53-dependent apoptosis as well as a global reduction of RNA polymerase I, II and III transcripts. In Wdr74-deficient embryos blocking Trp53 function rescues blastocyst formation and lineage differentiation. These results indicate that Wdr74 is required for RNA transcription, processing and/or stability during preimplantation development and is an essential gene in the mouse.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0022516