ENZO: a web tool for derivation and evaluation of kinetic models of enzyme catalyzed reactions

We describe a web tool ENZO (Enzyme Kinetics), a graphical interface for building kinetic models of enzyme catalyzed reactions. ENZO automatically generates the corresponding differential equations from a stipulated enzyme reaction scheme. These differential equations are processed by a numerical so...

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Bibliographic Details
Published in:PloS one 2011-07, Vol.6 (7), p.e22265-e22265
Main Authors: Bevc, Staš, Konc, Janez, Stojan, Jure, Hodošček, Milan, Penca, Matej, Praprotnik, Matej, Janežič, Dušanka
Format: Article
Language:English
Subjects:
Acetylcholinesterase - metabolism
Algorithms
Animals
Biocatalysis - drug effects
Biology
Butyrylthiocholine - pharmacology
Catalytic Domain
Cathepsin B - metabolism
Chemical reactions
Computer Science
Coordinate measuring machines
Differential equations
Drosophila melanogaster
Engineering
Enzyme Activation - drug effects
Enzyme kinetics
Enzymes
Enzymes - metabolism
Evaluation
Insects
Internet
Kinetics
Mathematical models
Mathematics
Models, Biological
Numerical analysis
Ordinary differential equations
Reaction kinetics
Reference Standards
Substrate Specificity - drug effects
Titrimetry
Torpedo - metabolism
User interface
Web applications
Web site management software
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Summary:We describe a web tool ENZO (Enzyme Kinetics), a graphical interface for building kinetic models of enzyme catalyzed reactions. ENZO automatically generates the corresponding differential equations from a stipulated enzyme reaction scheme. These differential equations are processed by a numerical solver and a regression algorithm which fits the coefficients of differential equations to experimentally observed time course curves. ENZO allows rapid evaluation of rival reaction schemes and can be used for routine tests in enzyme kinetics. It is freely available as a web tool, at http://enzo.cmm.ki.si.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0022265