Imaging of zebrafish in vivo with second-harmonic generation reveals shortened sarcomeres associated with myopathy induced by statin

We employed second-harmonic generation (SHG) imaging and the zebrafish model to investigate the myopathy caused by statin in vivo with emphasis on the altered microstructures of the muscle sarcomere, the fundamental contractile element of muscles. This approach derives an advantage of SHG imaging to...

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Bibliographic Details
Published in:PloS one 2011-09, Vol.6 (9), p.e24764
Main Authors: Huang, Shih-Hao, Hsiao, Chung-Der, Lin, Dar-Shong, Chow, Cho-Yen, Chang, Chia-Jen, Liau, Ian
Format: Article
Language:English
Subjects:
Animals
Biocompatibility
Biology
Biomarkers
Cholesterol
Cholesterol - metabolism
Consolidation
Coronary vessels
Creatine
Creatine kinase
Danio rerio
Disease
Dose-Response Relationship, Drug
Enzymes
Female
Hydroxymethylglutaryl-CoA Reductase Inhibitors - toxicity
Image Processing, Computer-Assisted - methods
Imaging
In vivo methods and tests
Injuries
Larva - drug effects
Larva - growth & development
Larva - physiology
Male
Medicine
Mevalonic acid
Mevalonic Acid - metabolism
Microfibrils - drug effects
Microfibrils - physiology
Microscopy
Microscopy, Fluorescence, Multiphoton - methods
Microstructure
Morphology
Motility
Muscle contraction
Muscle Contraction - drug effects
Muscle proteins
Muscle, Skeletal - drug effects
Muscle, Skeletal - metabolism
Muscle, Skeletal - physiopathology
Muscles
Muscular Diseases - chemically induced
Muscular Diseases - metabolism
Muscular Diseases - physiopathology
Musculoskeletal system
Myocytes
Myopathy
Myosin
Nanoparticles
Pathogenesis
Patients
Rodents
Sample preparation
Sarcomeres
Sarcomeres - physiology
Science
Skeletal muscle
Statins
Time Factors
Veterinary Science
Zebrafish
Zebrafish - growth & development
Zebrafish - physiology
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Description
Summary:We employed second-harmonic generation (SHG) imaging and the zebrafish model to investigate the myopathy caused by statin in vivo with emphasis on the altered microstructures of the muscle sarcomere, the fundamental contractile element of muscles. This approach derives an advantage of SHG imaging to observe the striated skeletal muscle of living zebrafish based on signals produced mainly from the thick myosin filament of sarcomeres without employing exogenous labels, and eliminates concern about the distortion of muscle structures caused by sample preparation in conventional histological examination. The treatment with statin caused a significantly shortened sarcomere relative to an untreated control (1.73±0.09 µm vs 1.91±0.08 µm, P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0024764