PS integrins and laminins: key regulators of cell migration during Drosophila embryogenesis

• Published in: PloS one 2011-09, Vol.6 (9), p.e23893-e23893
• Main Authors: Urbano, Jose M, Domínguez-Giménez, Paloma, Estrada, Beatriz, Martín-Bermudo, María D
• Format: Article
• Language: English
• Subjects: Animals; Animals, Genetically Modified; Biology; Cell adhesion & migration; Cell migration; Cell Movement; Drosophila; Drosophila - embryology; Drosophila - genetics; Drosophila - metabolism; Drosophila melanogaster; Drosophila Proteins - genetics; Drosophila Proteins - metabolism; Embryo, Nonmammalian - cytology; Embryo, Nonmammalian - embryology; Embryo, Nonmammalian - metabolism; Embryogenesis; Embryonic development; Embryonic growth stage; Embryos; Endoderm; Extracellular matrix; Gene expression; Glands; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; In Situ Hybridization; Insects; Integrins; Integrins - genetics; Integrins - metabolism; Kinases; Laminin; Laminin - genetics; Laminin - metabolism; Mammals; Mesoderm; Mesoderm - cytology; Mesoderm - embryology; Mesoderm - metabolism; Microscopy, Confocal; Morphogenesis; Mutation; Presenilin 1; Presenilin 2; Regulators; Salivary glands; Time-Lapse Imaging; Trachea; Vertebrates
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0023893

During embryonic development, there are numerous cases where organ or tissue formation depends upon the migration of primordial cells. In the Drosophila embryo, the visceral mesoderm (vm) acts as a substrate for the migration of several cell populations of epithelial origin, including the endoderm, the trachea and the salivary glands. These migratory processes require both integrins and laminins. The current model is that αPS1βPS (PS1) and/or αPS3βPS (PS3) integrins are required in migrating cells, whereas αPS2βPS (PS2) integrin is required in the vm, where it performs an as yet unidentified function. Here, we show that PS1 integrins are also required for the migration over the vm of cells of mesodermal origin, the caudal visceral mesoderm (CVM). These results support a model in which PS1 might have evolved to acquire the migratory function of integrins, irrespective of the origin of the tissue. This integrin function is highly specific and its specificity resides mainly in the extracellular domain. In addition, we have identified the Laminin α1,2 trimer, as the key extracellular matrix (ECM) component regulating CVM migration. Furthermore, we show that, as it is the case in vertebrates, integrins, and specifically PS2, contributes to CVM movement by participating in the correct assembly of the ECM that serves as tracks for migration.