Hippocampal neurogenesis and dendritic plasticity support running-improved spatial learning and depression-like behaviour in stressed rats

• Published in: PloS one 2011-09, Vol.6 (9), p.e24263-e24263
• Main Authors: Yau, Suk-Yu, Lau, Benson Wui-Man, Tong, Jian-Bin, Wong, Richard, Ching, Yick-Pang, Qiu, Guang, Tang, Siu-Wa, Lee, Tatia M C, So, Kwok-Fai
• Format: Article
• Language: English
• Subjects: Aging; Animals; Biology; Body Weight - physiology; Brain-derived neurotrophic factor; Brain-Derived Neurotrophic Factor - metabolism; Cell growth; Cell Proliferation; Corticosterone; Dendrites - physiology; Dendritic plasticity; Dendritic spines; Depression (Mood disorder); Depression - genetics; Depression - physiopathology; Doublecortin protein; Hippocampal plasticity; Hippocampus; Hippocampus - cytology; Hippocampus - metabolism; Immunohistochemistry; In Vitro Techniques; Labeling; Labelling; Laboratories; Male; Maze learning; Maze Learning - physiology; Medicine; Mental depression; Neurogenesis; Neurogenesis - physiology; Neuronal Plasticity - physiology; Neurons; Physical Conditioning, Animal - physiology; Physical training; Plastic properties; Plasticity; Pyramidal cells; Rats; Rats, Sprague-Dawley; Rodents; Runners (Sports); Running; Science; Spatial discrimination learning; Spine; Stress; Stresses; Thymidine
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0024263

Exercise promotes hippocampal neurogenesis and dendritic plasticity while stress shows the opposite effects, suggesting a possible mechanism for exercise to counteract stress. Changes in hippocampal neurogenesis and dendritic modification occur simultaneously in rats with stress or exercise; however, it is unclear whether neurogenesis or dendritic remodeling has a greater impact on mediating the effect of exercise on stress since they have been separately examined. Here we examined hippocampal cell proliferation in runners treated with different doses (low: 30 mg/kg; moderate: 40 mg/kg; high: 50 mg/kg) of corticosterone (CORT) for 14 days. Water maze task and forced swim tests were applied to assess hippocampal-dependent learning and depression-like behaviour respectively the day after the treatment. Repeated CORT treatment resulted in a graded increase in depression-like behaviour and impaired spatial learning that is associated with decreased hippocampal cell proliferation and BDNF levels. Running reversed these effects in rats treated with low or moderate, but not high doses of CORT. Using 40 mg/kg CORT-treated rats, we further studied the role of neurogenesis and dendritic remodeling in mediating the effects of exercise on stress. Co-labelling with BrdU (thymidine analog) /doublecortin (immature neuronal marker) showed that running increased neuronal differentiation in vehicle- and CORT-treated rats. Running also increased dendritic length and spine density in CA3 pyramidal neurons in 40 mg/kg CORT-treated rats. Ablation of neurogenesis with Ara-c infusion diminished the effect of running on restoring spatial learning and decreasing depression-like behaviour in 40 mg/kg CORT-treated animals in spite of dendritic and spine enhancement. but not normal runners with enhanced dendritic length. The results indicate that both restored hippocampal neurogenesis and dendritic remodelling within the hippocampus are essential for running to counteract stress.