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Increased oxidative stress induces apoptosis in human cystic fibrosis cells
Oxidative stress results in deleterious cell function in pathologies associated with inflammation. Here, we investigated the generation of superoxide anion as well as the anti-oxidant defense systems related to the isoforms of superoxide dismutases (SOD) in cystic fibrosis (CF) cells. Pro-apoptotic...
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Published in: | PloS one 2011-09, Vol.6 (9), p.e24880 |
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description | Oxidative stress results in deleterious cell function in pathologies associated with inflammation. Here, we investigated the generation of superoxide anion as well as the anti-oxidant defense systems related to the isoforms of superoxide dismutases (SOD) in cystic fibrosis (CF) cells. Pro-apoptotic agents induced apoptosis in CF but not in control cells that was reduced by treatment with SOD mimetic. These effects were associated with increased superoxide anion production, sensitive to the inhibition of IκB-α phosphorylation, in pancreatic but not tracheal CF cells, and reduced upon inhibition of either mitochondrial complex I or NADPH oxidase. CF cells exhibited reduced expression, but not activity, of both Mn-SOD and Cu/Zn-SOD when compared to control cells. Although, expression of EC-SOD was similar in normal and CF cells, its activity was reduced in CF cells. We provide evidence that high levels of oxidative stress are associated with increased apoptosis in CFTR-mutated cells, the sources being different depending on the cell type. These observations underscore a reduced anti-oxidant defense mechanism, at least in part, via diminished EC-SOD activity and regulation of Cu/Zn-SOD and Mn-SOD expressions. These data point to new therapeutic possibilities in targeting anti-oxidant pathways to reduce oxidative stress and apoptosis in CF cells. |
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Here, we investigated the generation of superoxide anion as well as the anti-oxidant defense systems related to the isoforms of superoxide dismutases (SOD) in cystic fibrosis (CF) cells. Pro-apoptotic agents induced apoptosis in CF but not in control cells that was reduced by treatment with SOD mimetic. These effects were associated with increased superoxide anion production, sensitive to the inhibition of IκB-α phosphorylation, in pancreatic but not tracheal CF cells, and reduced upon inhibition of either mitochondrial complex I or NADPH oxidase. CF cells exhibited reduced expression, but not activity, of both Mn-SOD and Cu/Zn-SOD when compared to control cells. Although, expression of EC-SOD was similar in normal and CF cells, its activity was reduced in CF cells. We provide evidence that high levels of oxidative stress are associated with increased apoptosis in CFTR-mutated cells, the sources being different depending on the cell type. These observations underscore a reduced anti-oxidant defense mechanism, at least in part, via diminished EC-SOD activity and regulation of Cu/Zn-SOD and Mn-SOD expressions. These data point to new therapeutic possibilities in targeting anti-oxidant pathways to reduce oxidative stress and apoptosis in CF cells.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0024880</identifier><identifier>PMID: 21931865</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acetophenones - pharmacology ; Allopurinol - pharmacology ; Anions ; Apoptosis ; Apoptosis - drug effects ; Biology ; Blotting, Western ; Cardiomyocytes ; Cell culture ; Cell Line, Tumor ; Copper ; Cystic fibrosis ; Cystic Fibrosis - metabolism ; Dactinomycin - pharmacology ; Electron Spin Resonance Spectroscopy ; Electron transport chain ; Endoplasmic reticulum ; Enzymes ; Homeostasis ; Humans ; Inflammation ; Inhibition ; Intensive care ; Isoforms ; Life Sciences ; Manganese ; Medicine ; Mitochondria ; Mutation ; NAD(P)H oxidase ; NADH-ubiquinone oxidoreductase ; NADPH Oxidases - metabolism ; Neutrophils ; Oxidases ; Oxidative stress ; Oxidative Stress - drug effects ; Pancreas ; Pancreatic cancer ; Pathogens ; Phosphorylation ; Proteins ; Smooth muscle ; Superoxide ; Superoxide Dismutase - metabolism ; Superoxides ; Tumor necrosis factor-TNF ; Xanthine Oxidase - metabolism ; Zinc</subject><ispartof>PloS one, 2011-09, Vol.6 (9), p.e24880</ispartof><rights>COPYRIGHT 2011 Public Library of Science</rights><rights>2011 Rottner et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Here, we investigated the generation of superoxide anion as well as the anti-oxidant defense systems related to the isoforms of superoxide dismutases (SOD) in cystic fibrosis (CF) cells. Pro-apoptotic agents induced apoptosis in CF but not in control cells that was reduced by treatment with SOD mimetic. These effects were associated with increased superoxide anion production, sensitive to the inhibition of IκB-α phosphorylation, in pancreatic but not tracheal CF cells, and reduced upon inhibition of either mitochondrial complex I or NADPH oxidase. CF cells exhibited reduced expression, but not activity, of both Mn-SOD and Cu/Zn-SOD when compared to control cells. Although, expression of EC-SOD was similar in normal and CF cells, its activity was reduced in CF cells. We provide evidence that high levels of oxidative stress are associated with increased apoptosis in CFTR-mutated cells, the sources being different depending on the cell type. These observations underscore a reduced anti-oxidant defense mechanism, at least in part, via diminished EC-SOD activity and regulation of Cu/Zn-SOD and Mn-SOD expressions. These data point to new therapeutic possibilities in targeting anti-oxidant pathways to reduce oxidative stress and apoptosis in CF cells.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>21931865</pmid><doi>10.1371/journal.pone.0024880</doi><tpages>e24880</tpages><orcidid>https://orcid.org/0000-0003-3897-7397</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Acetophenones - pharmacology Allopurinol - pharmacology Anions Apoptosis Apoptosis - drug effects Biology Blotting, Western Cardiomyocytes Cell culture Cell Line, Tumor Copper Cystic fibrosis Cystic Fibrosis - metabolism Dactinomycin - pharmacology Electron Spin Resonance Spectroscopy Electron transport chain Endoplasmic reticulum Enzymes Homeostasis Humans Inflammation Inhibition Intensive care Isoforms Life Sciences Manganese Medicine Mitochondria Mutation NAD(P)H oxidase NADH-ubiquinone oxidoreductase NADPH Oxidases - metabolism Neutrophils Oxidases Oxidative stress Oxidative Stress - drug effects Pancreas Pancreatic cancer Pathogens Phosphorylation Proteins Smooth muscle Superoxide Superoxide Dismutase - metabolism Superoxides Tumor necrosis factor-TNF Xanthine Oxidase - metabolism Zinc |
title | Increased oxidative stress induces apoptosis in human cystic fibrosis cells |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-13T06%3A01%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Increased%20oxidative%20stress%20induces%20apoptosis%20in%20human%20cystic%20fibrosis%20cells&rft.jtitle=PloS%20one&rft.au=Rottner,%20Mathilde&rft.date=2011-09-12&rft.volume=6&rft.issue=9&rft.spage=e24880&rft.pages=e24880-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0024880&rft_dat=%3Cgale_plos_%3EA476880187%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c725t-d28b31a06b32554d0499a81d882a5b6a118fd4cf28c3df2f36cfc922af078daf3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1308912884&rft_id=info:pmid/21931865&rft_galeid=A476880187&rfr_iscdi=true |