Hypervirulent K. pneumoniae secretes more and more active iron-acquisition molecules than "classical" K. pneumoniae thereby enhancing its virulence

A new hypervirulent (hypermucoviscous) clinical variant of Klebsiella pneumoniae (hvKP) has emerged over the last decade. Our goal is to identify new mechanisms, which increase the virulence hvKP compared to "classic" K. pneumoniae (cKP). Various growth assays were performed in human ascit...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2011-10, Vol.6 (10), p.e26734-e26734
Main Authors: Russo, Thomas A, Shon, Alyssa S, Beanan, Janet M, Olson, Ruth, MacDonald, Ulrike, Pomakov, Alexander O, Visitacion, Mark P
Format: Article
Language:English
Subjects:
Animals
Ascites
Bacilli
Bacillus anthracis
Bacterial infections
Bioassays
Biological assays
Biology
Cancer metastasis
Comparative analysis
Culture Media, Conditioned
E coli
Escherichia coli
Genotype & phenotype
Gram-negative bacilli
Human performance
Infections
Iron
Iron - metabolism
Klebsiella
Klebsiella pneumoniae
Klebsiella pneumoniae - metabolism
Klebsiella pneumoniae - pathogenicity
Liver
Medicine
Meningitis
Metastases
Metastasis
Mice
Models, Animal
Moraxella catarrhalis
Mortality
Pathogenesis
Pathogens
Pneumonia
Quorum Sensing
Resistance factors
Secretion
Siderophores
Survival
Virulence
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A new hypervirulent (hypermucoviscous) clinical variant of Klebsiella pneumoniae (hvKP) has emerged over the last decade. Our goal is to identify new mechanisms, which increase the virulence hvKP compared to "classic" K. pneumoniae (cKP). Various growth assays were performed in human ascites, human serum, and laboratory medium with the hvKP strain hvKP1 (wt), randomly chosen blood isolates of cKP strains (cKP1-4), and mutant constructs deficient in the secretion of selected compounds. An in vivo mouse model that mimics infection due to hvKP and a quantitative siderophore assay were also used. It was established that a molecule(s)/factor(s) was secreted by hvKP1 significantly enhanced its growth and/or survival in human ascites. This molecule(s)/factor(s) also increased the growth and/or survival of hvKP1 in serum ex vivo and in an in vivo mouse model that measures metastatic spread after subcutaneous challenge, thereby further establishing biologic significance. Although features such as a size of
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0026734