From SOMAmer-based biomarker discovery to diagnostic and clinical applications: a SOMAmer-based, streamlined multiplex proteomic assay

Recently, we reported a SOMAmer-based, highly multiplexed assay for the purpose of biomarker identification. To enable seamless transition from highly multiplexed biomarker discovery assays to a format suitable and convenient for diagnostic and life-science applications, we developed a streamlined,...

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Bibliographic Details
Published in:PloS one 2011-10, Vol.6 (10), p.e26332-e26332
Main Authors: Kraemer, Stephan, Vaught, Jonathan D, Bock, Christopher, Gold, Larry, Katilius, Evaldas, Keeney, Tracy R, Kim, Nancy, Saccomano, Nicholas A, Wilcox, Sheri K, Zichi, Dom, Sanders, Glenn M
Format: Article
Language:English
Subjects:
Assaying
Automation
Biological Assay - methods
Biology
Biomarkers
Biomarkers - analysis
Case-Control Studies
Deoxyribonucleic acid
Diagnostic software
Diagnostic systems
Diagnostic Techniques and Procedures
DNA
DNA polymerase
Humans
Hybridization
Laboratories
Ligands
Limit of Detection
Lung cancer
Medical diagnosis
Medicine
Multiplexing
Nucleic Acids - metabolism
Oligonucleotides - chemistry
Oligonucleotides - metabolism
Population
Proteins
Proteomics
Proteomics - methods
Reproducibility of Results
Therapeutic applications
Titrimetry
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Summary:Recently, we reported a SOMAmer-based, highly multiplexed assay for the purpose of biomarker identification. To enable seamless transition from highly multiplexed biomarker discovery assays to a format suitable and convenient for diagnostic and life-science applications, we developed a streamlined, plate-based version of the assay. The plate-based version of the assay is robust, sensitive (sub-picomolar), rapid, can be highly multiplexed (upwards of 60 analytes), and fully automated. We demonstrate that quantification by microarray-based hybridization, Luminex bead-based methods, and qPCR are each compatible with our platform, further expanding the breadth of proteomic applications for a wide user community.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0026332